Urine Bacterial Community Convergence through Fertilizer Production: Storage, Pasteurization, and Struvite Precipitation
| Autor: | Ting Luo, Diana S. Aga, Heather E. Goetsch, Sarah-Jane Haig, Jose Jimenez, Kim Nace, Kirtana Ramadugu, Abraham Noe-Hays, Krista R. Wigginton, Nancy G. Love, Rebecca Lahr, Betsy Foxman, Charles Bott |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Struvite 030106 microbiology Pasteurization Urine 010501 environmental sciences engineering.material 01 natural sciences Microbiology law.invention 03 medical and health sciences chemistry.chemical_compound law RNA Ribosomal 16S Environmental Chemistry Humans Food science Fertilizers 0105 earth and related environmental sciences biology Bacteria Lactobacillales Clostridiales General Chemistry biology.organism_classification Microbial population biology chemistry engineering Fertilizer |
| Zdroj: | Environmental sciencetechnology. 50(21) |
| ISSN: | 1520-5851 |
| Popis: | Source-separated human urine was collected from six public events to study the impact of urine processing and storage on bacterial community composition and viability. Illumina 16S rRNA gene sequencing revealed a complex community of bacteria in fresh urine that differed across collection events. Despite the harsh chemical conditions of stored urine (pH > 9 and total ammonia nitrogen > 4000 mg N/L), bacteria consistently grew to 5 ± 2 × 108 cells/mL. Storing hydrolyzed urine for any amount of time significantly reduced the number of operational taxonomic units (OTUs) to 130 ± 70, increased Pielou evenness to 0.60 ± 0.06, and produced communities dominated by Clostridiales and Lactobacillales. After 80 days of storage, all six urine samples from different starting materials converged to these characteristics. Urine pasteurization or struvite precipitation did not change the microbial community, even when pasteurized urine was stored for an additional 70 days. Pasteurization decreased metabolic activity by ... |
| Databáze: | OpenAIRE |
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