Global metabolite profiles of rice brown planthopper-resistant traits reveal potential secondary metabolites for both constitutive and inducible defenses
Autor: | Yun Xu, Umaporn Uawisetwathana, Theerayut Toojinda, Wintai Kamolsukyeunyong, Thapakorn Somboon, Nitsara Karoonuthaisiri, Apichart Vanavichit, Royston Goodacre, Christopher T. Elliott, Olivier P. Chevallier, Intawat Nookaew |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
LC-HRMS
Metabolite Brown planthopper resistance Endocrinology Diabetes and Metabolism Flavonoid Clinical Biochemistry Secondary Metabolism Oryza sativa medicine.disease_cause urologic and male genital diseases Biochemistry Microbiology Hemiptera chemistry.chemical_compound Multi-block principal component analysis Metabolite profiling SDG 3 - Good Health and Well-being Gene Expression Regulation Plant Infestation medicine Metabolome Animals Chromatography High Pressure Liquid Disease Resistance chemistry.chemical_classification Thai Jasmine rice biology Phenylpropanoid urogenital system food and beverages Oryza biology.organism_classification Phenotype chemistry Dydrogesterone Brown planthopper Phloem |
Zdroj: | METABOLOMICS Uawisetwathana, U, Chevallier, O P, Xu, Y, Kamolsukyeunyong, W, Nookaew, I, Somboon, T, Toojinda, T, Vanavichit, A, Goodacre, R, Elliott, C T & Karoonuthaisiri, N 2019, ' Global metabolite profiles of rice brown planthopper-resistant traits reveal potential secondary metabolites for both constitutive and inducible defenses ', Metabolomics, vol. 15, no. 12, 151 . https://doi.org/10.1007/s11306-019-1616-0 |
DOI: | 10.1007/s11306-019-1616-0 |
Popis: | Introduction: Brown planthopper (BPH) is a phloem feeding insect that causes annual disease outbreaks, called hopper burn in many countries throughout Asia, resulting in severe damage to rice production. Currently, mechanistic understanding of BPH resistance in rice plant is limited, which has caused slow progression on developing effective rice varieties as well as effective farming practices against BPH infestation. Objective: To reveal rice metabolic responses during 8 days of BPH attack, this study examined polar metabolome extracts of BPH-susceptible (KD) and its BPH-resistant isogenic line (IL308) rice leaves. Methods: Ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS) was combined with multi-block PCA to analyze potential metabolites in response to BPH attack. Results: This multivariate statistical model revealed different metabolic response patterns between the BPH-susceptible and BPH-resistant varieties during BPH infestation. The metabolite responses of the resistant IL308 variety occurred on Day 1, which was significantly earlier than those of the susceptible KD variety which showed an induced response by Days 4 and 8. BPH infestation caused metabolic perturbations in purine, phenylpropanoid, flavonoid, and terpenoid pathways. While found in both susceptible and resistant rice varieties, schaftoside (1.8 fold), iso-schaftoside (1.7 fold), rhoifolin (3.4 fold) and apigenin 6-C-α-l-arabinoside-8-C-β-l-arabinoside levels (1.6 fold) were significantly increased in the resistant variety by Day 1 post-infestation. 20-hydroxyecdysone acetate (2.5 fold) and dicaffeoylquinic acid (4.7 fold) levels were considerably higher in the resistant rice variety than those in the susceptible variety, both before and after infestation, suggesting that these secondary metabolites play important roles in inducible and constitutive defenses against the BPH infestation. Conclusions: These potential secondary metabolites will be useful as metabolite markers and/or bioactive compounds for effective and durable approaches to address the BPH problem. |
Databáze: | OpenAIRE |
Externí odkaz: |
načítá se...