Alterations of Choline Phospholipid Metabolism in Endometrial Cancer Are Caused by Choline Kinase Alpha Overexpression and a Hyperactivated Deacylation Pathway
| Autor: | Patrizia Lee, Eric O. Aboagye, Rohini Sharma, Hector C. Keun, David J. Pinato, Roberto Dina, James K. Ellis, Sebastian Trousil |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
medicine.medical_specialty Choline kinase Phosphorylcholine Choline kinase alpha Adenocarcinoma Biology Cell Line Choline Malignant transformation chemistry.chemical_compound Cell Line Tumor Internal medicine medicine Choline Kinase Humans Phospholipids Phosphocholine Hyperplasia Endometrial cancer Middle Aged Lipid Metabolism medicine.disease Endometrial Neoplasms Endometrial hyperplasia Choline transporter Endocrinology Oncology chemistry Phospholipases Cancer research Female Thiolester Hydrolases Signal Transduction |
| Zdroj: | Cancer Research. 74:6867-6877 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/0008-5472.can-13-2409 |
| Popis: | Metabolic rearrangements subsequent to malignant transformation are not well characterized in endometrial cancer. Identification of altered metabolites could facilitate imaging-guided diagnosis, treatment surveillance, and help to identify new therapeutic options. Here, we used high-resolution magic angle spinning magnetic resonance mass spectroscopy on endometrial cancer surgical specimens and normal endometrial tissue to investigate the key modulators that might explain metabolic changes, incorporating additional investigations using qRT-PCR, Western blotting, tissue microarrays (TMA), and uptake assays of [3H]-labeled choline. Lipid metabolism was severely dysregulated in endometrial cancer with various amino acids, inositols, nucleobases, and glutathione also altered. Among the most important lipid-related alterations were increased phosphocholine levels (increased 70% in endometrial cancer). Mechanistic investigations revealed that changes were not due to altered choline transporter expression, but rather due to increased expression of choline kinase α (CHKA) and an activated deacylation pathway, as indicated by upregulated expression of the catabolic enzymes LYPLA1, LYPLA2, and GPCPD1. We confirmed the significance of CHKA overexpression on a TMA, including a large series of endometrial hyperplasia, atypical hyperplasia, and adenocarcinoma tissues, supporting a role for CHKA in malignant transformation. Finally, we documented several-fold increases in the uptake of [3H]choline in endometrial cancer cell lines compared with normal endometrial stromal cells. Our results validate deregulated choline biochemistry as an important source of noninvasive imaging biomarkers for endometrial cancer. Cancer Res; 74(23); 6867–77. ©2014 AACR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|