The sperm mitochondria-specific translocator has a key role in maternal mitochondrial inheritance
| Autor: | Hiroaki Hazama, Shigeru Kohno, Katsuhisa Omagari, Kenji Hayashida, Jun-ichi Masuda, Yoshiko Kadokawa, Kazuo Ohba |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
Non-Mendelian inheritance Mitochondrial DNA endocrine system medicine.medical_treatment TIM/TOM complex Mitochondrion Biology DNA Mitochondrial Mitochondrial Proteins Tom complex Mice GTP-Binding Proteins medicine Animals Antigens reproductive and urinary physiology Mice Inbred ICR In vitro fertilisation urogenital system Elimination factor tpis Antibodies Monoclonal Proteins Embryo Cell Biology General Medicine Molecular biology Sperm Spermatozoa Cell biology Protein Transport Genes Mitochondrial Sperm factor Female Spermatogenesis Maternal mitochondrial inheritance |
| Zdroj: | Cell biology international. 29(6):472-481 |
| ISSN: | 1065-6995 |
| Popis: | The mechanism of maternal mitochondrial inheritance in animals involves the selective elimination of sperm mitochondria by the elimination factor of the egg and the sperm mitochondria-specific factor. In vitro fertilization using sperm from isogenic mice incorporating heterospecific mitochondrial DNA (mtDNA) showed that the number of PCR positives of sperm mtDNA in two-cell embryos was significantly increased following sperm incubation with anti-tetratricopeptide repeat-containing protein involved in spermatogenesis (tpis) protein, anti-translocator of mitochondrial outer membrane (Tom) 22 and anti-Tom40 antibodies. The treatment of fertilized eggs with EGTA and other endonuclease inhibitors increased the sperm mtDNA levels. We conclude that the elimination factor, which is probably an endonuclease, is selectively received by the tpis protein of the sperm mitochondrial outer membrane within the egg. It is then transported into the sperm mitochondria by Tom22 and Tom40, where it destroys the sperm mtDNA, establishing the maternal inheritance of mtDNA. The Version of Record (VoR) is available at http://www.cellbiolint.org Cell biology international, 29(6), pp.472-481; 2005 |
| Databáze: | OpenAIRE |
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