Zp4 is completely dispensable for fertility in female rats†
| Autor: | Pan-Pan Long, Ming-Hua Zeng, Tian-Ying Li, Dan Guo, Hong-Wen Deng, Jun-Ting Yang, Hong-Mei Xiao, Hua-Lin Huang, Ying Sun, Chao Lv, Hang-Jin Tan, Le Wang, Ru-Ping Quan, Yan Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Infertility media_common.quotation_subject medicine.medical_treatment Fertility Biology Oogenesis Zona Pellucida Glycoproteins Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization medicine Animals Mating Zona pellucida Ovulation media_common Gene Editing 030219 obstetrics & reproductive medicine In vitro fertilisation Cell Biology General Medicine medicine.disease Rats 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Fertilization Female |
| Zdroj: | Biology of reproduction. 104(6) |
| ISSN: | 1529-7268 |
| Popis: | Zona pellucida (ZP), which is composed of at most four extracellular glycoproteins (ZP1, ZP2, ZP3, and ZP4) in mammals, shelters the oocytes and is vital in female fertility. Several studies have identified the indispensable roles of ZP1–3 in maintaining normal female fertility. However, the understanding of ZP4 is still very poor because only one study on ZP4-associated infertility performed in rabbits has been reported up to date. Here we investigated the function of mammalian Zp4 by creating a knockout (KO) rat strain (Zp4−/− rat) using CRISPR–Cas9-mediated DNA-editing method. The influence of Zp4 KO on ZP morphology and some pivotal processes of reproduction, including oogenesis, ovulation, fertilization, and pup production, were studied using periodic acid–Schiff’s staining, superovulation, in vitro fertilization, and natural mating. The ZP morphology in Zp4−/− rats was normal, and none of these pivotal processes was affected. This study renewed the knowledge of mammalian Zp4 by suggesting that Zp4 was completely dispensable for female fertility. |
| Databáze: | OpenAIRE |
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