Isolation and Characterization of Spore‐Forming Bacilli (SFB) from Shepherd's Purse (Capsella bursa‐pastoris)
| Autor: | Wonjun Lee, Keun-Sung Kim, Hye-Bin Kim |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Bacilli 030106 microbiology Bacillus cereus Bacillus Enterotoxin Polymerase Chain Reaction Microbiology Enterotoxins Hemolysin Proteins 03 medical and health sciences Paenibacillus Species Specificity Republic of Korea Vegetables Humans Food microbiology Capsella Spores Bacterial biology Brevibacillus Drug Resistance Microbial biology.organism_classification Europe 030104 developmental biology Genes Bacterial Bacillus pseudomycoides Food Microbiology Food Science |
| Zdroj: | Journal of Food Science. 81:M684-M691 |
| ISSN: | 1750-3841 0022-1147 |
| DOI: | 10.1111/1750-3841.13231 |
| Popis: | Shepherd's purse (Capsella bursa-pastoris), native to Europe, is commonly consumed fresh and sometimes inadequately washed before consumption in Korea. The objective of this study was to characterize isolates of spore-forming bacilli (SFB) in samples of fresh Shepherd's purse. Three genera were identified: Bacillus (9 species), Paenibacillus (3 species), and Brevibacillus (1 species). None of the genes of the hemolysin BL (HBL) and nonhemolytic enterotoxin (NHE) complexes, or of the emetic toxin, was detected in the 25 SFB isolates, except for 2 Bacillus pseudomycoides isolates, where all 3 genes of the HBL enterotoxin complex were detected. There were significant sequence variations between the 2 species (Bacillus cereus and B. pseudomycoides) in the 3 genes of the HBL enterotoxin complex. These findings may provide insights into the diverse characteristics of the B. pseudomycoides HBL enterotoxin complex. Antibiotic resistance was assessed using 8 antibiotics. Among the 25 SFB isolates, 11 showed resistance to antibiotics, of which 5 were multiresistant. Assessment of the spoilage potential showed that all 25 SFB isolates could produce enzymes that can cause spoilage of foods. In conclusion, our findings may serve as integrative information for food research and industrial sectors. |
| Databáze: | OpenAIRE |
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