Arsenic and Benzo[a]pyrene Differentially Alter the Capacity for Differentiation and Growth Properties of Primary Human Epidermal Keratinocytes
| Autor: | D. S. Perez, Raymond S. H. Yang, J. A. Campain, M. H. Fox, L. Armstrong-Lea |
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| Rok vydání: | 2003 |
| Předmět: |
Keratinocytes
Cellular differentiation Biology Toxicology Arsenic chemistry.chemical_compound Benzo(a)pyrene Image Processing Computer-Assisted medicine Humans Cells Cultured Carcinogen Ploidies Dose-Response Relationship Drug Arsenic toxicity Cell growth Cell Cycle Cell Differentiation DNA Cell cycle Flow Cytometry In vitro Cell biology medicine.anatomical_structure chemistry Biochemistry Carcinogens Calcium Keratinocyte Cell Division |
| Zdroj: | Toxicological Sciences. 76:280-290 |
| ISSN: | 1096-0929 |
| DOI: | 10.1093/toxsci/kfg232 |
| Popis: | Normal human epidermal keratinocytes (NHEK) have been chosen as an in vitro model to test the hypothesis that chemicals which alter or interfere in cellular differentiation will concomitantly induce growth perturbations and are, thus, potential carcinogens. In these studies, we have focused on two known skin carcinogens, arsenic and benzo(a)pyrene (BaP). Our results demonstrated that BaP inhibits terminal differentiation in NHEK, as measured by cross-linked envelope (CLE) formation, up to 5.8-fold in control and 1.7-fold in calcium (Ca2+)-treated cells. In comparison, arsenic decreased CLE formation 20-fold in control cells and 5.5-fold in Ca2+-treated NHEK. To characterize the effects of these agents on the growth rate and cell cycle distributions of NHEK, flow cytometric analysis was used. BaP at 2 microM increased proliferation rates by 29%. Altered cell-cycle distribution in BaP-treated cells indicated a more rapid progression through the cell cycle, possibly by a shortened G2 phase. In contrast, arsenic at 5 microM inhibited proliferation by 25%; growth arrest (9%) was also observed in NHEK treated with 2 mM Ca2+. Our findings suggest that, although both BaP and arsenic inhibit CLE production in NHEK, different mechanisms may be involved. Studies in progress will attempt to identify molecular markers involved in the observed chemical effects. These markers will facilitate a mechanistic understanding of how an altered balance between growth and differentiation may play a role in the transformation process in NHEK. |
| Databáze: | OpenAIRE |
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