Tomato spotted wilt virus L RNA encodes a putative RNA polymerase
| Autor: | R.W. Goldbach, Richard Kormelink, R. de O. Resende, Dick Peters, F. van Poelwijk, P. de Haan |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Bunyaviridae
viruses Molecular Sequence Data Restriction Mapping Laboratory of Virology RNA-dependent RNA polymerase Plant Viruses Laboratorium voor Virologie Open Reading Frames chemistry.chemical_compound Transcription (biology) Sequence Homology Nucleic Acid Virology RNA polymerase RNA polymerase I Life Science Amino Acid Sequence Cloning Molecular Polymerase Gene Library Genetics Base Sequence biology Intron RNA RNA virus DNA-Directed RNA Polymerases Blotting Northern biology.organism_classification chemistry DNA Viral biology.protein RNA Viral |
| Zdroj: | Journal of General Virology 71 (1991) Journal of General Virology, 71, 2207-2216 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-72-9-2207 |
| Popis: | The complete nucleotide sequence of the large (L) genome segment of tomato spotted wilt virus (TSWV) has been determined. The RNA is 8897 nucleotides long and contains complementary 3' and 5' ends, comprising 62 nucleotides at the 5' end and 66 nucleotides at the 3' end. The RNA is of negative polarity, with one large open reading frame (ORF) located on the viral complementary strand. This ORF corresponds to a primary translation product of 2875 amino acids in length, with a predicted Mr of 331,500. Comparison with the polymerase proteins of other negative-strand viruses indicates that this protein most likely represents the viral polymerase. The genetic organization of TSWV L RNA is similar to that of the L RNA segments of Bunyamwera and Hantaan viruses, animal-infecting representatives of the Bunyaviridae. |
| Databáze: | OpenAIRE |
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