Anti-IgE induces the opening of non selective cation channels on human basophils
| Autor: | I. Inoue, Jacques Benveniste, T. Shimahara, Francis Beauvais |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Patch-Clamp Techniques
chemical and pharmacologic phenomena Inflammation Basophil In Vitro Techniques Immunoglobulin E Antibodies Ion Channels chemistry.chemical_compound parasitic diseases medicine Humans Pharmacology (medical) Patch clamp Ion channel Pharmacology biology Cell Membrane hemic and immune systems Transmembrane protein Cell biology Basophils medicine.anatomical_structure chemistry Immunology biology.protein Liberation medicine.symptom Histamine |
| Zdroj: | Fundamentalclinical pharmacology. 8(3) |
| ISSN: | 0767-3981 |
| Popis: | Basophils play a major role in allergic reactions-particularly in late phase reactions-by releasing histamine and other mediators of inflammation. Although transmembrane ion fluxes are thought to play an important role in the modulation of histamine release, little is known about ion pathways through the basophil membrane. We thus studied human basophils from normal subjects (n = 25 cells) with the patch-clamp method. We observed that IgE-dependent activation of human basophils led to the opening of non selective cation channels with a 20pS conductance. This was obtained when the patch pipette was applied onto the cell surface and sealed onto it in order to measure transmembrane currents on a small surface of intact basophils (cell-attached configuration). Non selective channels with the same 20pS conductance were also observed when a membrane patch was detached from basophil and its inner side placed in a Ca(2+)-containing medium (inside-out configuration). These data are a first contribution of the patch-clamp method in the understanding of ion movements in human basophils. |
| Databáze: | OpenAIRE |
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