Comparative frequencies of HIV low-level viremia between real-time viral load assays at clinically relevant thresholds
| Autor: | Tri D. Do, Ann Butcher, John Duncan, Teri Liegler |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Adolescent Concordance Human immunodeficiency virus (HIV) HIV Infections Viremia Biology medicine.disease_cause Sensitivity and Specificity Young Adult Virology Low level viremia medicine Humans Quantitation Range Reverse Transcriptase Polymerase Chain Reaction Disease progression Reproducibility of Results virus diseases Middle Aged Viral Load medicine.disease United States CD4 Lymphocyte Count Infectious Diseases Real-time polymerase chain reaction Disease Progression HIV-1 RNA Viral Female Reagent Kits Diagnostic Viral load |
| Zdroj: | Journal of Clinical Virology. 52:S83-S89 |
| ISSN: | 1386-6532 |
| Popis: | The introduction of new real-time PCR HIV-1 assays with higher sensitivity and broader dynamic range has resulted in detection of low-level viremia (LLV) (≥ 50 copies/mL) in some patients who previously had undetectable HIV-1 viral load (VL) (50 copies/mL) with end-point PCR assays. It is therefore important to compare the performance of end-point and newer real-time PCR assays at medically relevant decision points.The study compared the results obtained with the end-point COBAS(®) AMPLICOR HIV-1 MONITOR Test, v1.5 to those obtained by three real-time PCR assays COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HIV-1 Test; the COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HIV-1 Test, v2.0; and the Abbott RealTime™ HIV-1 test.A total of 391 plasma specimens from HIV-1-infected patients from three US cities were tested with all four assays. The correlation and concordance of results between real-time and end-point PCR assays were calculated.There was a consistent and similar proportion (11.8-14.0%) of HIV-1 VL ≥ 50 copies/mL with the three real-time PCR assays for specimens recording50 copies/mL on the end-point PCR assay. The real-time PCR assays correlated with the end-point PCR assay within generally accepted limits, but consistently quantified higher than the end-point PCR assay between 50 and 200 copies/mL. Discrepancies in results were associated with patient CD4+ cell count and antiviral medication class.The clinical interpretation of VL results from real-time PCR assays should take into account their higher sensitivity at the lower quantitation range when assessing patients for disease progression and monitoring response to therapy in HIV-1-infected patients, in line with current treatment guidelines. |
| Databáze: | OpenAIRE |
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