Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport
| Autor: | Blaine Bisel, Joachim Seemann, Yanzhuang Wang, Jen Hsuan Wei, Danming Tang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Science
Golgi Apparatus Mitosis Biochemistry Coat Protein Complex I symbols.namesake Cell Biology/Membranes and Sorting Humans Secretory pathway Multidisciplinary Chemistry Vesicle Golgi Matrix Proteins Membrane Proteins COPI COP-Coated Vesicles Golgi apparatus Transport protein Cell biology Protein Transport Golgi cisterna symbols Medicine Research Article |
| Zdroj: | PLoS ONE, Vol 3, Iss 2, p e1647 (2008) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The Golgi apparatus in mammalian cells is composed of flattened cisternae that are densely packed to form stacks. We have used the Golgi stacking protein GRASP65 as a tool to modify the stacking state of Golgi cisternae. We established an assay to measure protein transport to the cell surface in post-mitotic cells in which the Golgi was unstacked. Cells with an unstacked Golgi showed a higher transport rate compared to cells with stacked Golgi membranes. Vesicle budding from unstacked cisternae in vitro was significantly increased compared to stacked membranes. These results suggest that Golgi cisternal stacking can directly regulate vesicle formation and thus the rate of protein transport through the Golgi. The results further suggest that at the onset of mitosis, unstacking of cisternae allows extensive and rapid vesiculation of the Golgi in preparation for its subsequent partitioning. |
| Databáze: | OpenAIRE |
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