Protective effect of aPhyllanthus orbicularisaqueous extract against UVB light in human cells
| Autor: | Carlos Frederico Martins Menck, Maribel González-Pumariega, Marioly Vernhes, Luciana Nogueira de Sousa Andrade, Keronninn M. Lima-Bessa, André Passaglia Schuch, Angel Sánchez-Lamar |
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| Rok vydání: | 2012 |
| Předmět: |
Time Factors
DNA Repair Ultraviolet Rays DNA repair Pharmaceutical Science Apoptosis Human skin Pyrimidine dimer Context (language use) Biology Cell Line Flow cytometry Colony-Forming Units Assay Drug Discovery Botany medicine Humans Clonogenic assay Fibroblast Pharmacology integumentary system medicine.diagnostic_test Plant Extracts Cuba MICROBIOLOGIA General Medicine Flow Cytometry Molecular biology Phyllanthus medicine.anatomical_structure Complementary and alternative medicine Pyrimidine Dimers Cell culture Molecular Medicine |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.3109/13880209.2012.695800 |
| Popis: | One approach to protect human skin against the dangerous effects of solar ultraviolet (UV) irradiation is the use of natural products, such as photoprotectors. Phyllanthus orbicularis Kunth (Euphorbiaceae) is a Cuban endemic plant used in popular medicine. Its antigenotoxicity effect against some harmful agents has been investigated. However, the effect in ultraviolet B (UVB)-irradiated human cells has not been previously assessed.The protective effect of a P. orbicularis extract against UVB light-induced damage in human cells was evaluated.DNA repair proficient (MRC5-SV) and deficient (XP4PA, complementation group XPC) cell-lines were used. Damaging effects of UVB light were evaluated by clonogenic assay and apoptosis induction by flow cytometry techniques. The extent of DNA repair itself was determined by the removal of cyclobutane pyrimidine dimers (CPDs). The CPDs were detected and quantified by slot-blot assay.Treatment of UVB-irradiated MRC5-SV cells with P. orbicularis extract increased the percentage of colony-forming cells from 36.03 ± 3.59 and 4.42 ± 1.45 to 53.14 ± 8.8 and 14.52 ± 1.97, for 400 and 600 J/m(2), respectively. A decrease in apoptotic cell population was observed in cells maintained within the extract. The P. orbicularis extract enhanced the removal of CPD from genomic DNA. The CPDs remaining were found to be about 27.7 and 1.1%, while with plant extract, treatment these values decreased to 16.1 and 0.2%, for 3 and 24 h, respectively.P. orbicularis aqueous extract protects human cells against UVB damage. This protective effect is through the modulation of DNA repair effectiveness. |
| Databáze: | OpenAIRE |
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