EGFR/erb-1, HER2/erb-2, CK7, LP34, Ki67 and p53 expression in preneoplastic lesions of bronchial epithelium
| Autor: | Lina Carvalho, Lia Teixeira, Maria Reis Silva, Vitor Sousa, Ana Alarcão, Maria João d’Aguiar, Joana Espírito Santo, Ana Gomes, Patrícia Couceiro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Polysomy
Pathology medicine.medical_specialty Proliferation index biology business.industry lcsh:R Chromogranin A lcsh:Medicine General Medicine medicine.disease medicine.disease_cause Basal Cell Hyperplasia General Biochemistry Genetics and Molecular Biology Squamous metaplasia Cytokeratin Dysplasia Poster Presentation biology.protein Medicine lcsh:Q business Carcinogenesis lcsh:Science |
| Zdroj: | BMC Proceedings BMC Proceedings, Vol 4, Iss Suppl 2, p P64 (2010) |
| ISSN: | 1753-6561 |
| Popis: | A prognostic interpretation of preneoplastic lesions would have impact in bronchial carcinoma early diagnosis and through the study of Erb-B family receptors as they have an important role in lung carcinogenesis. The existence of drugs as tirosine kinase inhibitors (TKis) stressed the importance of studying gene alterations for selected chemoprevention schemes and characterization of carcinogenesis. Bronchial preneoplastic lesions were characterized by immunohistochemistry using the antibodies LP34 (high weigh molecular cytokeratin), CK7, Chromogranin A, Ki67, p53, C-erbB-2 and EGFR. HER2 and EGFR gene copy number was also evaluated by fluorescent in situ hybridization (FISH) in those lesions. The expected results defined the origin cell for basal cell hyperplasia and squamous metaplasia as adaptative lesions and dysplasia. By known experiences and published data, beyond the stem cell, the spectral evolution of bronchial preneoplastic lesions was demonstrated by characterizing basal cells (LP34) and their neoplastic potentiality. Dysplasias showed a higher expression of EGFR, Ki67 and p53 with a stepwise increase with the gravity of the respective grading. C-erbB-2 immunohistochemical overexpression was a rare event in preneoplastic lesions. Polysomy was the main mechanism for EGFR and HER2/neu higher gene copy number and together with increased proliferation index (Ki67) will account to preview bronchial carcinogenesis. |
| Databáze: | OpenAIRE |
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