Cadmium in bone cement induces necrosis and decreases the viability of residual osteosarcoma cells: A xenograft study
| Autor: | Melek Aydın, Safiye Aktaş, Ömer Bekçioğlu, Hasan Havitcioglu, Ayse Pinar Ercetin Ozdemir, Nihat Demirhan Demirkıran |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis Cell Survival medicine.medical_treatment Apoptosis Bone Neoplasms Mice 03 medical and health sciences 0302 clinical medicine lcsh:Orthopedic surgery medicine Animals Humans Orthopedics and Sports Medicine Saline Cells Cultured Osteosarcoma 030222 orthopedics business.industry Bone Cements 030229 sport sciences General Medicine Bone cement medicine.disease Xenograft Model Antitumor Assays In vitro Disease Models Animal lcsh:RD701-811 Cell culture Surgery Tumor necrosis factor alpha Neoplasm Recurrence Local medicine.symptom business Cadmium Research Article |
| Zdroj: | Acta Orthopaedica et Traumatologica Turcica, Vol 54, Iss 4, Pp 445-452 (2020) Acta Orthop Traumatol Turc |
| Popis: | Objective The aim of this study was to show whether local application of cadmium-impregnated bone cement can induce apoptosis and decrease the viability of residual osteosarcoma (OS) cells in nude mice. Methods K7M2 tumorigenic OS cell line was cultivated in vitro. The xenograft tumor model was formed by subcutaneously adding the tumor cells to athymic nude mice. Tumor was formed within 1 month. Then, mice were randomly assigned to five groups, each containing seven nude mice: control (group 1), wide resection (group 2), intralesional resection (group 3), intralesional resection + bone cement (group 4), and intralesional resection + cadmium embedded in bone cement (group 5). Tumor resection with 1 cm surgical margins was performed in the wide resection group. In intralesional resection groups, tumor tissue was resected with positive margins aiming to leave 15 mm3 of macroscopic tumor tissue. In group 3, the defect was left empty; groups 4 and 5 received bone cements prepared with saline and cadmium solutions, respectively. After the resection, mice were observed for 15 days and sacrificed. Next, surgical resection sites were evaluated histopathologically in each group. Results Recurrent tumor was formed in all mice in the wide resection group, and apparent progression of residual tumor was observed in groups 3 and 4. On the contrary, only a thin layer of residual tumor was observed around the bone cement in group 5. Histological evaluation revealed remarkable necrosis in group 5 and lowest viability compared to other groups. No systemic toxic effect related to cadmium was observed. Conclusion Our data suggest that local application of cadmium in bone cement has a significant potential to increase tumor necrosis and decrease the viability of residual OS cells. |
| Databáze: | OpenAIRE |
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