Characterisation of Peste Des Petits Ruminants Disease in Pastoralist Flocks in Ngorongoro District of Northern Tanzania and Bluetongue Virus Co-Infection
| Autor: | Mark Henstock, Brian Clarke, Chobi Chubwa, Richard Kock, Carrie Batten, Satya Parida, Mana Mahapatra, Julius Keyyu, Hayley Hicks, Bryony A. Jones |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary medicine sheep goats 040301 veterinary sciences ethno-veterinary knowledge Pastoralism lcsh:QR1-502 Disease Antibodies Viral Bluetongue Tanzania Rinderpest PPR Article Virus lcsh:Microbiology Disease Outbreaks Peste-des-petits-ruminants virus Diagnosis Differential 0403 veterinary science 03 medical and health sciences Virology Peste-des-Petits-Ruminants parasitic diseases differential diagnosis Animals Phylogeny biology Coinfection 04 agricultural and veterinary sciences biology.organism_classification outbreak investigation 030104 developmental biology Infectious Diseases Animals Domestic surveillance RNA Viral Flock Bluetongue virus Co infection |
| Zdroj: | Viruses, Vol 12, Iss 389, p 389 (2020) Viruses Volume 12 Issue 4 |
| ISSN: | 1999-4915 |
| Popis: | Peste des petits ruminants (PPR) disease was first confirmed in Tanzania in 2008 in sheep and goats in Ngorongoro District, northern Tanzania, and is now endemic in this area. This study aimed to characterise PPR disease in pastoralist small ruminant flocks in Ngorongoro District. During June 2015, 33 PPR-like disease reports were investigated in different parts of the district, using semi-structured interviews, clinical examinations, PPR virus rapid detection test (PPRV-RDT), and laboratory analysis. Ten flocks were confirmed as PPRV infected by PPRV-RDT and/or real-time reverse transcription-polymerase chain reaction (RT-qPCR), and two flocks were co-infected with bluetongue virus (BTV), confirmed by RT-qPCR. Phylogenetic analysis of six partial N gene sequences showed that the PPR viruses clustered with recent lineage III Tanzanian viruses, and grouped with Ugandan, Kenyan and Democratic Republic of Congo isolates. No PPR-like disease was reported in wildlife. There was considerable variation in clinical syndromes between flocks: some showed a full range of PPR signs, while others were predominantly respiratory, diarrhoea, or oro-nasal syndromes, which were associated with different local disease names (olodua&mdash a term for rinderpest, olkipiei&mdash lung disease, oloirobi&mdash fever, enkorotik&mdash diarrhoea). BTV co-infection was associated with severe oro-nasal lesions. This clinical variability makes the field diagnosis of PPR challenging, highlighting the importance of access to pen-side antigen tests and multiplex assays to support improved surveillance and targeting of control activities for PPR eradication. |
| Databáze: | OpenAIRE |
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