Simultaneous determination of rutin and ascorbic acid in a sequential injection lab-at-valve system
| Autor: | Yuliia V. Miekh, Mohammed Khair E. A. Al-Shwaiyat, Yaroslav Bazel, Vasil Andruch, Tatyana A. Denisenko, Andriy B. Vishnikin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Anions
Calibration curve Chemistry Pharmaceutical Rutin Clinical Biochemistry Analytical chemistry Pharmaceutical Science Ascorbic Acid 02 engineering and technology Sensitivity and Specificity 01 natural sciences Analytical Chemistry Reaction rate chemistry.chemical_compound Limit of Detection Drug Discovery Phosphoric Acids Folin–Ciocalteu reagent Spectroscopy Molybdenum Detection limit Isosbestic point Chromatography 010401 analytical chemistry Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Ascorbic acid 0104 chemical sciences Drug Combinations chemistry Reagent Calibration Flow Injection Analysis Indicators and Reagents 0210 nano-technology Tablets |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 149:179-184 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2017.11.006 |
| Popis: | A green, simple, accurate and highly sensitive sequential injection lab-at-valve procedure has been developed for the simultaneous determination of ascorbic acid (Asc) and rutin using 18-molybdo-2-phosphate Wells-Dawson heteropoly anion (18-MPA). The method is based on the dependence of the reaction rate between 18-MPA and reducing agents on the solution pH. Only Asc is capable of interacting with 18-MPA at pH 4.7, while at pH 7.4 the reaction with both Asc and rutin proceeds simultaneously. In order to improve the precision and sensitivity of the analysis, to minimize reagent consumption and to remove the Schlieren effect, the manifold for the sequential injection analysis was supplemented with external reaction chamber, and the reaction mixture was segmented. By the reduction of 18-MPA with reducing agents one- and two-electron heteropoly blues are formed. The fraction of one-electron heteropoly blue increases at low concentrations of the reducer. Measurement of the absorbance at a wavelength corresponding to the isobestic point allows strictly linear calibration graphs to be obtained. The calibration curves were linear in the concentration ranges of 0.3–24 mg L −1 and 0.2–14 mg L −1 with detection limits of 0.13 mg L −1 and 0.09 mg L −1 for rutin and Asc, respectively. The determination of rutin was possible in the presence of up to a 20-fold molar excess of Asc. The method was applied to the determination of Asc and rutin in ascorutin tablets with acceptable accuracy and precision (1–2%). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect