Leishmania (Viannia) peruviana (MHOM/PE/LCA08): Comparison of THP-1 cell and murine macrophage susceptibility to axenic amastigotes for the screening of leishmanicidal compounds
| Autor: | Yannick Estevez, Denis Castillo, Eric Deharo, Thomas Grentzinger, German Gonzalez |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
Immunology Antiprotozoal Agents Leishmania braziliensis Monocytes Microbiology Inhibitory Concentration 50 Mice Amphotericin B Cell Line Tumor medicine Murine macrophages Animals Humans Macrophage Viability assay Amastigote Leishmania peruviana Leishmania Leishmanicidal biology Kinetoplastida Leishmaniasis General Medicine biology.organism_classification medicine.disease Infectious Diseases Cell culture Leukemia Monocytic Acute Macrophages Peritoneal THP-1 Female Parasitology |
| Zdroj: | Experimental Parasitology. 122:353-356 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2009.05.005 |
| Popis: | This study, undertaken to compare the susceptibility of THP-1 cells and murine peritoneal macrophages to Leishmania peruviana amastigotes, obtained THP-1 infection with 10 parasites/cell compared to 2 parasites/murine macrophage. The parasite burden was maximal at 72 h post-infection (h.p.i.) for THP-1 cells, while it was still increasing at 120 h.p.i. for murine macrophages. Since in both cases the infection with L. peruviana affected cell viability, we recommend evaluating any leishmanicidal activity at 72 h.p.i. Amphotericin B reduced Leishmania infection by 50% at concentrations of 0.1 microM in THP-1 and murine macrophages at 72 h.p.i. Our results demonstrate that amastigotes of L. peruviana can infect THP-1 cells and murine macrophages and indicate the suitability of this model to screen compounds for leishmanicidal activity. |
| Databáze: | OpenAIRE |
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