Novel components of the Apicomplexan moving junction reveal conserved and coccidia-restricted elements
| Autor: | Catherine S. Sohn, Stephen J. Cheng, Peter J. Bradley, Kurtis W. Straub |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Immunology Protozoan Proteins Biology Kidney Microbiology Plasmodium Article Cell Line Host-Parasite Interactions Cell membrane Apicomplexa Mice Coccidia Virology parasitic diseases medicine Extracellular Animals Humans Secretion Mice Inbred BALB C Cell Membrane Neospora Fibroblasts biology.organism_classification Cell biology medicine.anatomical_structure Rhoptry neck Cytoplasm Toxoplasma HeLa Cells |
| Popis: | Apicomplexan parasites generally invade their host cells by anchoring the parasite to the host membrane through a structure called the moving junction (MJ). This moving junction is also believed to sieve host proteins from the nascent parasitophorous vacuole membrane, which likely protects the pathogen from lysosomal destruction. Previously identified constituents of the Toxoplasma MJ have orthologues in Plasmodium, indicating a conserved structure throughout the Apicomplexa. We report here two novel MJ proteins, RON5 and RON8. While RON5 is conserved in Plasmodium, RON8 appears restricted to the coccidia. RON8, which is likely essential, coimmunoprecipitates RON5 and known MJ proteins from extracellular parasites, indicating a preformed complex exists within the parasites. Upon secretion, we show that RON8 within the MJ localizes to the cytoplasmic face of the host plasma membrane. To examine interactions between RON8 and the host cell, we expressed RON8 in mammalian cells and show that it targets to its site of action at the periphery in a manner dependent on the C-terminal portion of the protein. The discovery of RON5 and RON8 provides new insight into conserved and unique elements of the MJ, furthering our understanding of how the moving junction contributes to the intricate mechanism of Apicomplexan invasion. |
| Databáze: | OpenAIRE |
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