Polypeptides related to mammalian procholecystokinin in the brain of an invertebrate, a marine worm, Nereis diversicolor: Evidence from in Ovo translation of mRNA
| Autor: | Jean Jacques Curgy, Sanae Guissi-Kadri, Philippe Bulet |
|---|---|
| Přispěvatelé: | Université de Lille |
| Rok vydání: | 1990 |
| Předmět: |
Immunoprecipitation
[SDV]Life Sciences [q-bio] Xenopus Blotting Western Cross Reactions Biology In ovo Marine worm Epitope Endocrinology Protein biosynthesis Animals Humans RNA Messenger Protein Precursors Brain Chemistry Messenger RNA Neuropeptides Polychaeta biology.organism_classification Precipitin Tests Molecular biology Rats Blot Protein Biosynthesis Oocytes Electrophoresis Polyacrylamide Gel Female Animal Science and Zoology Cholecystokinin |
| Zdroj: | General and Comparative Endocrinology General and Comparative Endocrinology, 1990, 77 (3), pp.339-347. ⟨10.1016/0016-6480(90)90223-9⟩ |
| ISSN: | 0016-6480 1095-6840 |
| DOI: | 10.1016/0016-6480(90)90223-9 |
| Popis: | Total mRNA extracted from brain of a sea worm Nereis diversicolor (Annelida, Polychaeta) were injected into Xenopus oocytes. The in ovo-translated polypeptides were analyzed through electrophoresis of immunoprecipitated products or Western blotting techniques. Some of these polypeptides cross-reacted antibodies elaborated against three mammalian (human and rat) procholecystokinin (pro-CCK) sequences. Polypeptides of 15, 64, and 70 kDa were determined, but two families of less obvious products, whose molecular masses were between 27-34 kDa and 46-60 kDa, appeared. Furthermore, the Western blotting technique also showed a cross-reaction between some of the polypeptides (64 and 70 kDa) extracted from brains and the antibodies used. From this work, evidence is given of the presence of epitopes shared by cellular polypeptides extracted from the brain, in ovo-translated products and mammalian pro-CCK. Furthermore, these data suggest the existence of a CCK precursor in the brain of N. diversicolor. |
| Databáze: | OpenAIRE |
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