Comparison between two derivatization methods of nitrite ion labeled with 15N applied to liquid chromatography-tandem mass spectrometry
| Autor: | Yu Haiyan, Chaimbault Patrick, Leroy Pierre, Schmitt Romain, Sapin Anne |
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| Přispěvatelé: | Cibles thérapeutiques, formulation et expertise pré-clinique du médicament (CITHEFOR), Université de Lorraine (UL), Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes (LCP-A2MC) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Detection limit Azo compound Chromatography Ussing chamber General Chemical Engineering 010401 analytical chemistry General Engineering Tandem mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry Adduct 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Fragmentation (mass spectrometry) chemistry Liquid chromatography–mass spectrometry [CHIM]Chemical Sciences Derivatization ComputingMilieux_MISCELLANEOUS |
| Zdroj: | Analytical Methods Analytical Methods, Royal Society of Chemistry, 2018, 10 (31), pp.3830-3836. ⟨10.1039/C8AY01206G⟩ |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c8ay01206g |
| Popis: | The fragmentations of the adducts resulting from the derivatization of nitrite ion by Griess method (formation of an azo compound) and 2,3-diaminonaphthalene (formation of 2,3-naphthotriazole (NAT)) were studied by tandem mass spectrometry (MS/MS). The transition used for quantification was selected according to the highest value of the signal-over-blank ratio (S/B) obtained from each adduct fragmentation. When derivatizing nitrite ion labeled with the stable 15N isotope, followed by liquid chromatography (LC)-MS/MS measurement, the lowest limit of quantification obtained was 5 nM with NAT. The method was applied to study the intestinal permeability of S-nitrosoglutathione, a drug candidate for the chronic treatment of cardiovascular disease. This compound was labeled with 15N and its permeability was studied in an ex vivo rat intestine model using an Ussing chamber. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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