The effect of Toll-like receptor 4 gene polymorphism on vascular complications in type 2 diabetes patients

Autor: Monika Buraczynska, Piotr Wacinski, Katarzyna Ksiazek, Pawel Zukowski, Michal Dragan
Rok vydání: 2016
Předmět:
Adult
Male
0301 basic medicine
medicine.medical_specialty
Genotype
Endocrinology
Diabetes and Metabolism
Type 2 diabetes
030204 cardiovascular system & hematology
Diabetic angiopathy
Polymerase Chain Reaction
Polymorphism
Single Nucleotide
Gastroenterology
03 medical and health sciences
0302 clinical medicine
Endocrinology
Diabetic Neuropathies
Gene Frequency
Internal medicine
Diabetes mellitus
Odds Ratio
Internal Medicine
medicine
Humans
Diabetic Nephropathies
Genetic Predisposition to Disease
Allele frequency
Alleles
Aged
Diabetic Retinopathy
business.industry
Case-control study
General Medicine
Middle Aged
medicine.disease
Genotype frequency
Toll-Like Receptor 4
030104 developmental biology
Diabetes Mellitus
Type 2
Cardiovascular Diseases
Case-Control Studies
Female
Gene polymorphism
business
Diabetic Angiopathies
Zdroj: Diabetes Research and Clinical Practice. 116:7-13
ISSN: 0168-8227
DOI: 10.1016/j.diabres.2016.04.002
Popis: Objective The aim of our study was to assess the association between the TLR4 Asp299Gly polymorphism and vascular complications in patients with type 2 diabetes. Methods We examined 1090 patients with T2DM and 716 healthy controls. All subjects were genotyped for the Asp299Gly polymorphism by polymerase chain reaction (PCR) and restriction analysis. Results The genotype frequencies of the Asp299Gly polymorphism were similar in T2DM patients and controls ( p =0.512 and 0.311, respectively). The polymorphism was analyzed in subgroups of patients with macro- and microvascular complications. The distribution of genotypes was significantly different between patients with CVD and those without CVD. A significant increase of G allele frequency was observed in CVD+ patients, with odds ratio 2.06 (1.27–3.34), p =0.0035. The same effect was found when patients with diabetic retinopathy were compared with those without it (OR for G allele 2.12, 95% CI 1.43–3.12, p =0.0002). There were no statistically significant differences in genotype distribution between patients with diabetic nephropathy or neuropathy and those without these complications. Conclusions The results of our study demonstrated that the G allele of the Asp299Gly polymorphism of the TLR4 gene is associated with increased risk of cardiovascular disease and diabetic retinopathy in type 2 diabetes patients.
Databáze: OpenAIRE
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