3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

Autor:	Carol J. Hirschmugl, Jonathan M. Castro, Michael J. Nasse, Charlotte Dabat-Blondeau, Michael C. Martin, Hans A. Bechtel, David Buschke, Barbara L. Illman, Brenda M. Ogle, Miriam Unger, Julia Sedlmair, Marco Keiluweit, Dilworth Y. Parkinson
Rok vydání:	2013
Předmět:	Chemical imaging medicine.medical_specialty Materials science Infrared spectroscopy Biochemistry Fourier transform spectroscopy Physics::Geophysics Mice symbols.namesake Imaging Three-Dimensional Optics Spectroscopy Fourier Transform Infrared Microscopy Image Processing Computer-Assisted medicine Animals Humans Fourier transform infrared spectroscopy Molecular Biology Embryonic Stem Cells business.industry X-Ray Microtomography Cell Biology Wood Spectral imaging Populus Fourier transform symbols business Chemical fingerprinting Synchrotrons Hair Biotechnology
Zdroj:	Nature Methods. 10:861-864
ISSN:	1548-7105 1548-7091
DOI:	10.1038/nmeth.2596
Popis:	We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89efde0b7268ab2ef73343c72007a800 https://doi.org/10.1038/nmeth.2596 Zobrazit plný text záznamu Plný text ve formátu PDF