Carbohydrate content of apolipoprotein B-48 from rat chylomicrons of varying density
| Autor: | Regina Slowinska, Wieslaw J. Bochenek, Gexin Tang, Peter Weber, John B. Rodgers |
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| Rok vydání: | 1990 |
| Předmět: |
Male
Apolipoprotein B-48 Apolipoprotein B Carbohydrates Galactosamine 1-Propanol Biochemistry chemistry.chemical_compound Chylomicrons Centrifugation Density Gradient Animals Chemical Precipitation Monosaccharide Mesentery Centrifugation Triglycerides Apolipoproteins B chemistry.chemical_classification Glucosamine biology Triglyceride Chemistry digestive oral and skin physiology Organic Chemistry Galactose nutritional and metabolic diseases Rats Inbred Strains Cell Biology Carbohydrate Rats biology.protein Poloxalene lipids (amino acids peptides and proteins) Lymph Mannose Chylomicron |
| Zdroj: | Lipids. 25:665-668 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/bf02536019 |
| Popis: | Monosaccharide composition was determined in apolipoprotein B-48 (apoB) of chylomicrons of rat mesenteric lymph. Chylomicrons were separated into three fractions based on density. Triglyceride and apolipoprotein content were determined in each. ApoB was isolated and quantified using precipitation with isopropanol. Chylomicrons were collected in lymph under normal conditions, and with Poloxalene 2930 when chylomicron secretion was inhibited. Most of the triglyceride was carried in the least dense fraction, while the highest apoB content was in the most dense fraction under normal conditions. Mannose and galactosamine contents of apoB were similar in all fractions while contents of both glucosamine and galactose were highest in the least dense fraction. When chylomicron secretion was inhibited by Poloxalene, the amount of triglyceride recovered in the least dense fraction was significantly reduced. Despite the inhibition of lipid transport in the least dense fraction of chylomicrons by Poloxalene, there was little change in apoB recoveries and in the relative content of various monosaccharides in the apoB from each of the three fractions as compared to results obtained during lipid absorption under normal conditions. In conclusion, carbohydrate composition of apoB of chylomicrons is heterogeneous and varies with chylomicron density. |
| Databáze: | OpenAIRE |
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