Detection of Anaerobic Bacteria in High Numbers in Sputum from Patients with Cystic Fibrosis
| Autor: | Andrew McDowell, Richard C. Boucher, Thomas Fintan Moriarty, Marianne S. Muhlebach, J. Stuart Elborn, Tyler R. Field, Matthew C. Wolfgang, Michael M. Tunney, Sheila Patrick, Gerd Doering, Deirdre Gilpin |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Pulmonary and Respiratory Medicine Adolescent Cystic Fibrosis Veillonella Critical Care and Intensive Care Medicine Microbiology Bacteria Anaerobic Risk Factors Forced Expiratory Volume Intensive care medicine Prevotella Humans Child Bacteriological Techniques biology business.industry Sputum Bacterial Infections Middle Aged biology.organism_classification respiratory tract diseases Child Preschool Anaerobic bacteria medicine.symptom business Bronchoalveolar Lavage Fluid Anaerobic exercise Actinomyces Bacteria |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 177:995-1001 |
| ISSN: | 1535-4970 1073-449X |
| Popis: | Pulmonary infection in cystic fibrosis (CF) is polymicrobial and it is possible that anaerobic bacteria, not detected by routine aerobic culture methods, reside within infected anaerobic airway mucus.To determine whether anaerobic bacteria are present in the sputum of patients with CF.Sputum samples were collected from clinically stable adults with CF and bronchoalveolar lavage fluid (BALF) samples from children with CF. Induced sputum samples were collected from healthy volunteers who did not have CF. All samples were processed using anaerobic bacteriologic techniques and bacteria within the samples were quantified and identified.Anaerobic species primarily within the genera Prevotella, Veillonella, Propionibacterium, and Actinomyces were isolated in high numbers from 42 of 66 (64%) sputum samples from adult patients with CF. Colonization with Pseudomonas aeruginosa significantly increased the likelihood that anaerobic bacteria would be present in the sputum. Similar anaerobic species were identified in BALF from pediatric patients with CF. Although anaerobes were detected in induced sputum samples from 16 of 20 volunteers, they were present in much lower numbers and were generally different species compared with those detected in CF sputum. Species-dependent differences in the susceptibility of the anaerobes to antibiotics with known activity against anaerobes were apparent with all isolates susceptible to meropenem.A range of anaerobic species are present in large numbers in the lungs of patients with CF. If these anaerobic bacteria are contributing significantly to infection and inflammation in the CF lung, informed alterations to antibiotic treatment to target anaerobes, in addition to the primary infecting pathogens, may improve management. |
| Databáze: | OpenAIRE |
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