The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization
| Autor: | Rita de Cássia dos Santos Silveira, Andréa Lúcia Corso, Luiz Fernando Wurdig Roesch, Renato S. Procianoy, Priscila Caroline Thiago Dobbler, Volker Mai |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Estudo observacional
Microbial diversity Microbial DNA Técnicas de amplificação de ácido nucleico Physiology Gut flora Applied Microbiology and Biotechnology Pregnancy Lactobacillus RNA Ribosomal 16S Prevotella 0303 health sciences biology Microbiota General Medicine Biodiversity Europe Estudos transversais Vagina Female Análise de sequência de RNA Brazil Biotechnology Adult DNA Bacterial Microbioma gastrointestinal Disease cluster Vaginal microbiome 03 medical and health sciences Young Adult Next generation sequencing medicine Dominance (ecology) Humans 16S rRNA 030304 developmental biology Original Paper Bacteria 030306 microbiology Brasil Infant Newborn Recém-nascido medicine.disease biology.organism_classification United Kingdom Gastrointestinal Microbiome Microbial population biology Multivariate Analysis Gravidez RNA ribossômico 16S |
| Zdroj: | Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS World Journal of Microbiology & Biotechnology |
| Popis: | The female lower genital tract harbors a complex microbial community essential for homeostasis and health. During pregnancy, the female body undergoes unique hormonal changes that contribute to weight gain as well as modulations in immune function that can affect microbiota composition. Several studies have described the vaginal microbiota of pregnant women from the USA, Europe and Mexico. Here we expand our knowledge about the vaginal microbial communities during the third trimester to healthy expectant Brazilian mothers. Vaginal samples were collected from patients delivering at the Hospital de Clínicas de Porto Alegre, Brazil. Microbial DNA was isolated from samples and the V4 region of the 16S rRNA gene was amplified and sequenced using the PGM Ion Torrent. Brazilian pregnant women presented three distinct types of microbial community at the time of labor. Two microbial communities, Cluster 1 and Cluster 3, presented an overall dominance of Lactobacillus while Cluster 2 tended to present higher diversity and richness, with the presence of Pseudomonas, Prevotella and other vaginosis related bacteria. About half of the Brazilian mothers sampled here had dominance of L. iners. The proportion of mothers without dominance of any Lactobacillus was higher in Brazil (22%) compared to UK (2.4%) and USA, where this community type was not detected. The vaginal microbiota showed significant correlation with the composition of the babies’ gut microbiota (p-value = 0.002 with a R2 of 15.8%). Mothers presenting different vaginal microbiota shared different microorganisms with their newborns, which would reflect on initial colonizers of the developing newborns’ gut. |
| Databáze: | OpenAIRE |
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