Genomic binding of Pol III transcription machinery and relationship with TFIIS transcription factor distribution in mouse embryonic stem cells
| Autor: | Olivier Alibert, Janos Murvai, Lucie Carrière, Ivo G. Gut, Jean-Christophe Aude, Mark Lathrop, Fayçal Boussouar, Mario Foglio, Julie Soutourina, Yad Ghavi-Helm, Céline Keime, Matthieu Gérard, Sylvie Jounier, Marta Gut, Hélène Humbertclaude, Sébastien Graziani, Michel Werner |
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| Přispěvatelé: | Institut de Biologie et de Technologies de Saclay (IBITECS), Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire d'optique et biosciences (LOB), École polytechnique (X)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Exploration Fonctionnelle des Génomes (LEFG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7), INSERM U823, Institut Albert Bonniot, Laboratoire de Biologie Intégrative (LBI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Centre National de Génotypage (CNG), Parc Científic de Barcelona, c/Baldiri Reixac 4, Centro Nacional de Analisis Genomico [Barcelona] (CNAG), Centre Nacional D'Anàlisi Genómica, Parc Cientific de Barcelona, Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
| Rok vydání: | 2011 |
| Předmět: |
Chromatin Immunoprecipitation
Transcription Genetic DNA polymerase [SDV]Life Sciences [q-bio] RNA polymerase II RNA polymerase III Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine RNA Transfer Transcription Factor TFIIIB Transcription Factors TFIII Transcription (biology) RNA Small Nuclear RNA polymerase Genetics Animals Short Interspersed Nucleotide Elements Transcription factor Embryonic Stem Cells ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Binding Sites Genome biology Nuclear Proteins RNA Polymerase III RNA-Binding Proteins Genomics Sequence Analysis DNA Chromatin chemistry biology.protein RNA Polymerase II Transcriptional Elongation Factors Butyrate Response Factor 1 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2012, 40 (1), pp.270-283. ⟨10.1093/nar/gkr737⟩ Nucleic Acids Research, 2012, 40 (1), pp.270-283. ⟨10.1093/nar/gkr737⟩ |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | RNA polymerase (Pol) III synthesizes the tRNAs, the 5S ribosomal RNA and a small number of untranslated RNAs. In vitro, it also transcribes short interspersed nuclear elements (SINEs). We investigated the distribution of Pol III and its associated transcription factors on the genome of mouse embryonic stem cells using a highly specific tandem ChIP-Seq method. Only a subset of the annotated class III genes was bound and thus transcribed. A few hundred SINEs were associated with the Pol III transcription machinery. We observed that Pol III and its transcription factors were present at 30 unannotated sites on the mouse genome, only one of which was conserved in human. An RNA was associated with >80% of these regions. More than 2200 regions bound by TFIIIC transcription factor were devoid of Pol III. These sites were associated with cohesins and often located close to CTCF-binding sites, suggesting that TFIIIC might cooperate with these factors to organize the chromatin. We also investigated the genome-wide distribution of the ubiquitous TFIIS variant, TCEA1. We found that, as in Saccharomyces cerevisiae, TFIIS is associated with class III genes and also with SINEs suggesting that TFIIS is a Pol III transcription factor in mammals. |
| Databáze: | OpenAIRE |
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