Detection of Latent Peronospora effusa Infections in Spinach
| Autor: | Amy Anchieta, Sridhara G. Kunjeti, Nikhilesh Dhar, Chaitra S. Subbarao, Lorena Ochoa, Steven J. Klosterman, Krishna V. Subbarao |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Host resistance Peronospora food and beverages Plant Science Biology biology.organism_classification 01 natural sciences Fungicides Industrial Fungicide Plant Leaves 03 medical and health sciences Horticulture 030104 developmental biology Species Specificity Peronospora effusa Spinacia oleracea Downy mildew Spinach Agronomy and Crop Science 010606 plant biology & botany Plant Diseases |
| Zdroj: | Plant disease. 102(9) |
| ISSN: | 1943-7692 |
| Popis: | Downy mildew disease of spinach, caused by Peronospora effusa, is managed in conventional fields by a combination of host resistance and scheduled fungicide applications. Fungicides are currently applied to prevent downy mildew epidemics regardless of the infection status of spinach crops. A more streamlined approach would be to develop methods to target either latent infections for fungicide application in conventional production systems or to hasten harvest in organic production. In this study, conventional polymerase chain reaction (PCR) was applied to detect P. effusa DNA in symptomless spinach leaves in three spatially and temporally separated field plots, each containing four 2-m beds, 35 m in length. Spinach leaves were sampled weekly at 3-m intervals at 48 locations throughout each plot. Initial samples were asymptomatic and yet PCR enabled detection of P. effusa DNA extracted from sampled spinach leaves. Detection of latent downy mildew infection in spinach leaves was confirmed by PCR as early as 7 days prior to symptom development. The limit of pathogen DNA detection in spinach leaves was calculated at 10 pg using the conventional PCR approach. Quantitative PCR with TaqMan methodology revealed the presence of inhibitors from spinach leaf DNA extracts and affected amplification efficiencies, but not when diluted, enabling detection of P. effusa DNA at a concentration of |
| Databáze: | OpenAIRE |
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