Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition
| Autor: | Adam J. Moore, Khadidiatou Mangou, Bacary D. Sadio, Mariama N. Pouye, Seynabou D. Sene, Ousmane Faye, Alassane Mbengue, Amy K. Bei, Fatoumata Diallo |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Erythrocytes medicine.drug_class Science 030106 microbiology Plasmodium falciparum Protozoan Proteins Functional impact Single-nucleotide polymorphism Biology Monoclonal antibody Polymorphism Single Nucleotide Article 03 medical and health sciences medicine Parasite immune evasion Animals Humans Parasite genetics Allele Genetics Genetic diversity Multidisciplinary Malaria vaccine Merozoites Antibodies Monoclonal High-Throughput Nucleotide Sequencing Parasite biology 030104 developmental biology Basigin Medicine Carrier Proteins Ex vivo |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) |
| ISSN: | 2045-2322 |
| Popis: | The PfRh5-Basigin ligand–receptor interaction is an essential step in the merozoite invasion process and represents an attractive vaccine target. To reveal genotype–phenotype associations between naturally occurring allelic variants of PfRh5 and invasion inhibition, we performed ex vivo invasion inhibition assays with monoclonal antibodies targeting basigin coupled with PfRh5 next-generation amplicon sequencing. We found dose-dependent inhibition of invasion across all isolates tested, and no statistically significant difference in invasion inhibition for any single nucleotide polymorphisms. This study demonstrates that PfRh5 remains highly conserved and functionally essential, even in a highly endemic setting, supporting continued development as a strain-transcendent malaria vaccine target. |
| Databáze: | OpenAIRE |
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