Characterization and selection of Bacillus thuringiensis isolates effective against Sitophilus oryzae
| Autor: | Najara da Silva, Ana Maria Guidelli Thuler, Irlan Leite de Abreu, Manoel Victor Franco Lemos, Camila Chiaradia Davolos, Ricardo Antonio Polanczyk |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal do Espírito Santo (UFES) |
| Rok vydání: | 2010 |
| Předmět: |
Bactéria entomopatogênica
Biological pest control Virulence Biology Entomopathogenic bacterium Microbiology law.invention Curculionidae Curculionidade law Bacillus thuringiensis Botany Proteína cristal Polyacrylamide gel electrophoresis Polymerase chain reaction business.industry Sitophilus fungi crystal protein Pest control food and beverages biology.organism_classification Coleoptera Animal Science and Zoology PEST analysis business Agronomy and Crop Science |
| Zdroj: | SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0103-9016 |
| DOI: | 10.1590/s0103-90162010000400015 |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:01:15Z No. of bitstreams: 1 S0103-90162010000400015.pdf: 347357 bytes, checksum: ad8b87a041fbf2a38794adc9525e3c9a (MD5) Made available in DSpace on 2013-08-22T19:01:15Z (GMT). No. of bitstreams: 1 S0103-90162010000400015.pdf: 347357 bytes, checksum: ad8b87a041fbf2a38794adc9525e3c9a (MD5) Previous issue date: 2010-08-01 Made available in DSpace on 2013-09-30T20:01:03Z (GMT). No. of bitstreams: 2 S0103-90162010000400015.pdf: 347357 bytes, checksum: ad8b87a041fbf2a38794adc9525e3c9a (MD5) S0103-90162010000400015.pdf.txt: 34160 bytes, checksum: f356f8d63dec46cc01393244cbb74c07 (MD5) Previous issue date: 2010-08-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:13:10Z No. of bitstreams: 2 S0103-90162010000400015.pdf: 347357 bytes, checksum: ad8b87a041fbf2a38794adc9525e3c9a (MD5) S0103-90162010000400015.pdf.txt: 34160 bytes, checksum: f356f8d63dec46cc01393244cbb74c07 (MD5) Made available in DSpace on 2014-05-20T13:13:10Z (GMT). No. of bitstreams: 2 S0103-90162010000400015.pdf: 347357 bytes, checksum: ad8b87a041fbf2a38794adc9525e3c9a (MD5) S0103-90162010000400015.pdf.txt: 34160 bytes, checksum: f356f8d63dec46cc01393244cbb74c07 (MD5) Previous issue date: 2010-08-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) A bactéria entomopatogênica Bacillus thuringiensis (Bt) é um agente de controle com características tóxicas e ambientais que permitem o controle de insetos-praga de acordo com as premissas do Manejo integrado de pragas (MIP). Com o objetivo de buscar novas linhagens potencialmente tóxicas para Sitophilus oryzae L. 1763 (Coleoptera: Curculinidae), caracterizaram-se molecularmente 1,073 isolados de B. thuringiensis de regiões do Brasil. O material genético foi extraído através do kit InstaGene Matrix, utilizado para a amplificação das seqüências através da técnica de Polymerase chain reaction PCR, sendo os resultados visualizados em gel de agarose 1,5%. A classe do gene cry35Ba foi representada por 60 isolados (5,6%) de Bt, os quais foram submetidos a bioensaio com larvas de S. oryzae. Quatro causaram mortalidade acima de 50% nos testes de patogenicidade e os isolados 544 e 622 foram os mais virulentos, conforme determinado pela estimativa da CL50. Nos quatro isolados que demonstraram toxicidade, foram detectados cristais esféricos, bipiramidais e cubóides, além de proteínas com 44 kDa, referentes aos genes cry35Ba por Sodium dodecil sulphate - polyacrilamide gel electrophoresis (SDS-PAGE). Estes dados demonstram o potencial de Bt no manejo de S. oryzae. The entomopathogenic bacterium Bacillus thuringiensis is a control agent with toxic and environmental characteristics that allows the control of pest insects according to the Integrate Pest Management (IPM) precepts. In order to find new strains, potentially toxic to Sitophilus oryzae L. 1763 (Coleoptera: Curculinidae), 1.073 strains of B. thuringiensis from parts of Brazil were used. Genetic material was extracted with InstaGene Matrix kit, used for the amplification of sequences in Polymerase chain reaction (PCR), and viewed in 1.5% agarose gel. The gene cry35Ba class was represented by 60 B. thuringiensis isolates (5.6%), which were then subjected to bioassays with S. oryzae larvae. Among the isolates studied, four caused more than 50% mortality in pathogenicity tests, and the isolates 544 and 622 were the most virulent, as determined by CL50 estimates. The four toxic isolates had spherical, bi-pyramidal and cuboid crystals, and a 44-kDa protein was found in sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE), which coded for the product of cry35Ba genes. These data demonstrate the potential of B. thuringiensis for the management of S. oryzae larvae. UNESP FCAV Depto. de Biologia Aplicada à Agropecuária Universidade Federal do Espírito Santo (UFES) Depto. de Produção Vegetal UNESP FCAV Depto. de Biologia Aplicada à Agropecuária |
| Databáze: | OpenAIRE |
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