Identification of Coli Surface Antigen 23, a Novel Adhesin of Enterotoxigenic Escherichia coli
| Autor: | O. Colin Stine, Vsevolod L. Popov, Roberto Vidal, Myron M. Levine, Douglas J. Botkin, Patricio Valenzuela, Fernando Ruiz-Perez, Felipe Del Canto, James P. Nataro, Mihai Pop, Alfredo G. Torres |
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| Rok vydání: | 2012 |
| Předmět: |
Molecular Sequence Data
Immunology Fimbria Locus (genetics) medicine.disease_cause digestive system Microbiology Bacterial Adhesion Antigen Enterotoxigenic Escherichia coli medicine Humans Amino Acid Sequence ORFS Adhesins Bacterial Phylogeny Genetics Antigens Bacterial Base Sequence biology Escherichia coli Proteins Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition bacterial infections and mycoses Molecular Pathogenesis Bacterial adhesin Open reading frame Infectious Diseases Pilin Antigens Surface Mutation biology.protein Parasitology Caco-2 Cells |
| Zdroj: | INFECTION AND IMMUNITY Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00263-12 |
| Popis: | Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhea, mainly in developing countries. Although there are 25 different ETEC adhesins described in strains affecting humans, between 15% and 50% of the clinical isolates from different geographical regions are negative for these adhesins, suggesting that additional unidentified adhesion determinants might be present. Here, we report the discovery of Coli Surface Antigen 23 (CS23), a novel adhesin expressed by an ETEC serogroup O4 strain (ETEC 1766a), which was negative for the previously known ETEC adhesins, albeit it has the ability to adhere to Caco-2 cells. CS23 is encoded by an 8.8-kb locus which contains 9 open reading frames (ORFs), 7 of them sharing significant identity with genes required for assembly of K88-related fimbriae. This gene locus, named aal ( a dhesion- a ssociated l ocus), is required for the adhesion ability of ETEC 1766a and was able to confer this adhesive phenotype to a nonadherent E. coli HB101 strain. The CS23 major structural subunit, AalE, shares limited identity with known pilin proteins, and it is more closely related to the CS13 pilin protein CshE, carried by human ETEC strains. Our data indicate that CS23 is a new member of the diverse adhesin repertoire used by ETEC strains. |
| Databáze: | OpenAIRE |
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