Ultrastructure of a bio-electrolytic methanogenic/methanotrophic granular biofilm for the complete degradation of tetrachloroethylene in contaminated groundwater
| Autor: | M.-J. Levesque, Serge R. Guiot, Ruxandra Cimpoia, Ramona Kuhn |
|---|---|
| Přispěvatelé: | van Loosdrecht, M., Kruize, R., Lewandowski, Z. |
| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Tetrachloroethylene Environmental Engineering Hydraulic retention time Electron donor Dichloroethene Electrolysis environmental Bacteria Anaerobic chemistry.chemical_compound Bioreactors Bioremediation Water Supply RNA Ribosomal 16S Reductive dechlorination Effluent In Situ Hybridization Fluorescence Water Science and Technology Environmental engineering ultrastructure Dichloroethylenes Bacteria Aerobic chemistry Biofilms Environmental chemistry Methane Anaerobic exercise Water Pollutants Chemical |
| Zdroj: | Water Science and Technology. 55:465-471 |
| ISSN: | 1996-9732 0273-1223 |
| Popis: | The electrolytical methanogenic/methanotrophic coupling (eMaMoC) process was tested in a laboratory-scale single-stage reactor for the treatment of tetrachloroethene (PCE)-contaminated waters. A water electrolysis cell was placed directly in the effluent recirculation loop for the supply of both O2 and H2 to the system: H2 serving as the electron donor for both carbonate reduction into CH4 and reductive dechlorination. The concurrent presence of O2 and CH4 could be used by the methanotrophs for co-metabolically oxidising the chlorinated intermediates left over by the anaerobic transformation of PCE. At a PCE inlet of 33–52 μM and a hydraulic residence time (HRT) of 5.6 days, PCE reductive dechlorination to dichloroethene (DCE) was over 95% with a maximum DCE mineralisation of 83%. Fluorescence in situ hybridisation with 16S rRNA probes related to type I and type II methanotrophic bacteria were utilised to localise the methanotrophic communities in the anaerobic/aerobic granules. It evidenced that with operational time, along with increasing oxygenation rate, methanotrophic communities were specifically colonising onto the outermost layer of the anaerobic/aerobic granule. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|