Localization of the structural gene for human apolipoprotein A-I on the long arm of human chromosome 11
| Autor: | Martha Liao Law, Peter Pak-Hang Cheung, Theodore T. Puck, Lawrence Chan, Fa-Ten Kao, Carol Adaire Jones |
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| Rok vydání: | 1984 |
| Předmět: |
Chromosomes
Human 6-12 and X Genetics Multidisciplinary Apolipoprotein A-I Apolipoprotein B Human apolipoprotein Structural gene Chromosome Mapping Nucleic Acid Hybridization Chromosome Hamster Hybrid Cells Biology Molecular biology Apolipoproteins Cricetinae Complementary DNA biology.protein Animals Humans lipids (amino acids peptides and proteins) Chromosome Deletion Gene Research Article Southern blot |
| Zdroj: | Proceedings of the National Academy of Sciences. 81:508-511 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.81.2.508 |
| Popis: | Apolipoprotein A-I (apo A-I), the major apolipoprotein in human high density lipoproteins, is involved in the disease atherosclerosis. Cloned apo A-I cDNA (pA1-3) was used as a probe in chromosome mapping studies to detect the human apo A-I structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 13 hybrids localized the gene to human chromosome 11. Confirmation of the chromosomal assignment was obtained by analysis of a hybrid (J1) containing a single human chromosome, no. 11. Regional mapping was achieved by using deletion subclones of J1 that localized the human apo A-I structural gene to the region 11q13 leads to qter. Since the human apolipoprotein C-III (apo C-III) structural gene is closely linked to apo A-I, it can be assigned to the same region on the long arm of chromosome 11. By extension of methods previously described, it now appears possible to carry out fine-structure analysis of this and related gene regions on chromosome 11 and to study the biochemical concomitants of these genes and of genes on other chromosomes for analysis of their role in atherosclerosis. |
| Databáze: | OpenAIRE |
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