ω3PUFAs improve hepatic steatosis in postnatal overfed rats and HepG2 cells by inhibiting acetyl‐CoA carboxylase
Autor: | Yanyan Dai, Fan Yang, Susu Du, Nan Zhou, Xiaonan Li |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
chemistry.chemical_classification
medicine.medical_specialty postnatal overfeeding Nutrition. Foods and food supply Acetyl-CoA carboxylase Stimulation hepatic lipid synthesis medicine.disease Fish oil In vitro Oleic acid chemistry.chemical_compound Endocrinology chemistry Internal medicine Nonalcoholic fatty liver disease medicine lipids (amino acids peptides and proteins) TX341-641 Steatosis ACC ω3PUFAs Food Science Polyunsaturated fatty acid Original Research |
Zdroj: | Food Science & Nutrition, Vol 9, Iss 9, Pp 5153-5165 (2021) Food Science & Nutrition |
ISSN: | 2048-7177 |
Popis: | Postnatal overfeeding can lead to persistent increases in hepatic lipid synthesis and the risk of nonalcoholic fatty liver disease (NAFLD) in adulthood. The ω3 polyunsaturated fatty acids (ω3PUFAs) exhibit beneficial effects on NAFLD. Here, we employed a rat model and an in vitro HepG2 cell model to investigate whether fish oil (FO) affects hepatic lipid synthesis due to postnatal overfeeding. Male Sprague‐Dawley were divided into litter sizes of three (small litters, SLs) or 10 (normal litters, NLs) on postnatal day 3 and were fed standard chow or FO diet beginning on postnatal week 3 to generate NL, SL, NL‐FO, and SL‐FO groups. The results indicated that the FO diet reduced the postnatal overfeeding‐induced body weight gain and NAFLD characteristics (such as serum and liver triglyceride (TG) and hepatic steatosis). In addition, FO restored the expression of hepatic lipid metabolism‐related genes (including SCD1, FASN, CPT1, LPL, ACC, and SREBP‐1c) in SL‐FO rats. Specifically, the activity and expression pattern of ACC were consistent with SREBP‐1c. Furthermore, HepG2 cells were treated with oleic acid (OA), followed by eicosapentenoic acid (EPA), with or without SREBP‐1c siRNA. The cellular lipid droplets, TG content, and the expression of ACC (by 75%) and SREBP‐1c (by 45%) were increased by OA stimulation (p Post‐weaning fish oil diet could improve hepatic steatosis and decrease the risk of developing NAFLD in later life, which emphasizing that the ω3PUFAs supplementation should be encouraged in obese children, especially those who have experienced postnatal overfed. |
Databáze: | OpenAIRE |
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