Production of cachexia mediators by Walker 256 cells from ascitic tumors
| Autor: | Maria Eliane Merlin Rocha, Maria Benigna M. Oliveira, Glaucia Regina Martinez, Eva Gunilla Skare Carnieri, Guilhermina Rodrigues Noleto, Lívia Bracht, Rosilene Rebeca, Silvia Maria Suter Correia Cadena |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Cachexia Clinical Biochemistry Spermine Biology Nitric Oxide Biochemistry Dinoprostone Proinflammatory cytokine chemistry.chemical_compound Internal medicine Cell Line Tumor medicine Animals Ascitic Fluid Urea Prostaglandin E2 Carcinoma 256 Walker Rats Wistar Arginase Interleukin-6 Tumor Necrosis Factor-alpha Biogenic Polyamines Cell Biology General Medicine medicine.disease Rats Spermidine Endocrinology chemistry Cell culture Putrescine Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Cell biochemistry and function. 26(6) |
| ISSN: | 1099-0844 |
| Popis: | In neoplasic cachexia, chemical mediators seem to act as initiators or perpetuators of this process. Walker 256 cells, whose metabolic properties have so far been little studied with respect to cancer cachexia, are used as a model for the study of this syndrome. The main objective of this research was to pinpoint the substances secreted by these cells that may contribute to the progression of the cachectic state. Since inflammatory mediators seem to be involved in the manifestation of this syndrome, the in vitro production of nitric oxide (NO), cytokines (tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6)), and prostaglandin E2 (PGE2) was evaluated in Walker 256 cells isolated from ascitic tumors. After 4 or 5 h, a significant increase in NO production was observed (2.55 +/- 1.56 and 4.05 +/- 1.99 nmol NO per 10(7) cells, respectively). When isolated from a 6-day-old tumor, a significantly lower production of IL-6 and higher production of TNF-alpha than in cells from a 4-day-old tumor were observed, indicating a relationship between the production of cytokines and the time of tumor development after implantation. Considerable production of PGE(2) by Walker 256 cells isolated from the 6-day-old tumor was also observed. Polyamines were also determined in Walker 256 cells. Levels of putrescine, spermidine, and spermine did not show significant differences in tumors developed during 4 or 6 days. Direct evidence of the release of proinflammatory cytokines and PGE2 by Walker 256 cells suggests that these mediators can drive the cachectic syndrome in the host, the effect being dependent on tumor development time. |
| Databáze: | OpenAIRE |
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