Comparative Immunohistochemical Study of MUC1 and Carbohydrate Antigens in Breast Benign Disease and Normal Mammary Gland
| Autor: | Marina T. Isla-Larrain, Walter J. Servi, Sandra O. Demichelis, Amada Segal-Eiras, María Virginia Croce, Cecilio G. Alberdi |
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| Rok vydání: | 2010 |
| Předmět: |
Histology
Lewis X Antigen Breast Neoplasms Antibodies Epitope Pathology and Forensic Medicine chemistry.chemical_compound Antigen Biomarkers Tumor Humans Antigens Tumor-Associated Carbohydrate Mammary Glands Human MUC1 Principal Component Analysis biology Chemistry Mucin-1 Immunohistochemistry Molecular biology Medical Laboratory Technology Variable number tandem repeat Sialyl-Lewis X Polyclonal antibodies biology.protein Female Antibody |
| Zdroj: | Applied Immunohistochemistry & Molecular Morphology. 18:41-50 |
| ISSN: | 1541-2016 |
| DOI: | 10.1097/pai.0b013e3181ac1c20 |
| Popis: | The aim was to compare the expression of MUC1 and carbohydrate antigens in 124 tissue samples; 42 fibroadenoma (FA), 23 nonproliferative benign diseases (NPF), 25 usual epithelial hyperplasia (UEH), 7 atypical ductal hyperplasia (ADH), and 27 breast normal tissues. An immunohistochemical approach was adopted, using the following antibodies: reactive with MUC1 variable number of tandem repeats (C595, HMFG2, and SM3 monoclonal antibodies), anti-MUC1-cytoplasmic tail polyclonal antibody (CT33), and anti-carbohydrate antigens (sialyl Lewis x, Lewis x, Lewis y, Tn, and Thomsen-Friedenreich epitopes). Positive area of reaction, intensity, and pattern of expression were considered. A reactivity index was calculated as intensity (I) x 100+percentage of positive area (A). Statistical analysis comprised frequency analysis, P < 0.05, analysis of variance, and multiple correlation with principal component analysis. All samples expressed MUC1, detected by at least one anti-MUC1 antibody whereas Lewis x was the carbohydrate antigen most frequently found in all groups whereas variable number of tandem repeats MUC1 and Lewis x showed the highest correlation: 93% of normal samples, 62.5% of NPF, 87% of FA, 85% of UEH, and finally 80% of ADH. Although principal component analysis using reactivity indexes explained only 39% of data variability, normal samples appeared grouped and separated from benign breast diseases, which remained spread. Thomsen-Friedenreich was the only antigen that showed an increased tendency for positive expression and intensity from NPF through FA, UEH to ADH, whereas it was not detected in normals. With respect to the pattern of expression, an apical pattern was predominantly found in all the groups. |
| Databáze: | OpenAIRE |
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