Doxorubicin-induced alterations in kidney functioning, oxidative stress, DNA damage, and renal tissue morphology; Improvement by Acacia hydaspica tannin-rich ethyl acetate fraction
Autor: | Suhail Razak, Ali Almajwal, Tayyaba Afsar, Dara Al-Disi |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine γ-GT Gamma Glutamyl Transferase Antioxidant medicine.medical_treatment WBCs white blood cells Renal function Histopathology Pharmacology medicine.disease_cause 01 natural sciences Oxidative stress markers Article AHE Acacia hydaspica ethyl acetate extract Nephrotoxicity 03 medical and health sciences chemistry.chemical_compound Kidney function SOD superoxide dismutase medicine polycyclic compounds GR glutathione reductase Blood urea nitrogen lcsh:QH301-705.5 MDA malondialdehyde NO nitric oxide Kidney Creatinine GPx glutathione peroxidase QR quinone reductase DOX doxorubicin carbohydrates (lipids) 030104 developmental biology medicine.anatomical_structure chemistry lcsh:Biology (General) POD peroxidase GST glutathione S transferase Doxorubicin H2O2 hydrogen peroxide RBCs red blood cells Uric acid CAT catalase Genotoxicity General Agricultural and Biological Sciences Oxidative stress 010606 plant biology & botany |
Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 9, Pp 2251-2260 (2020) Saudi Journal of Biological Sciences |
Popis: | Doxorubicin (DOX) is an anthracycline drug used for cancer treatment. However, its treatment is contiguous with toxic effects. We examine the nephroprotective potential of A. hydaspica polyphenol-rich ethyl acetate extract (AHE) against DOX persuaded nephrotoxicity. 36 male Sprague Dawley rats were randomly assorted into 6 groups. Control group received saline; DOX group: 3 mg/kg b.w. dosage of DOX intraperitoneally for 6 weeks (single dose/week). In co-treatment groups, 200 and 400 mg/kg b.w AHE was given orally for 6 weeks in concomitant with DOX (3 mg/kg b.w, i.p. injection per week) respectively. Standard group received silymarin 400 mg/kg b.w daily + DOX (single dose/week). Biochemical kidney function tests, oxidative stress markers, genotoxicity, antioxidant enzyme status, and histopathological changes were examined. DOX caused significant body weight loss and decrease kidney weight. DOX-induced marked deterioration in renal function indicators in both urine and serum, i.e., PH, specific gravity, total protein, albumin, urea, creatinine, uric acid, globulin, blood urea nitrogen, etc. Also, DOX treatment increases renal tissue oxidative stress markers, while lower antioxidant enzymes in tissue along with degenerative alterations in the renal tissue compared to control rats. AHE co-treatment ameliorates DOX-prompted changes in serum and urine chemistry. Likewise, AHE treatment decreases sensitive markers of oxidative stress and prevented DNA damages by enhancing antioxidant enzyme levels. DOX induction in rats also caused DNA fragmentation which was restored by AHE co-treatment. Moreover, the histological observations evidenced that AHE effectively rescued the kidney tissue from DOXinterceded oxidative damage. Our results suggest that co-treatment of AHE markedly improve DOX-induced deleterious effects in a dose-dependent manner. The potency of AHE co-treatment at 400mg/kg dose is similar to silymarin. These outcomes revealed that A. hydaspica AHE extract might serve as a potential adjuvant that avoids DOX-induced nephrotoxicity. |
Databáze: | OpenAIRE |
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