Effect of valproic acid on miRNAs affecting histone deacetylase in a model of anaplastic thyroid cancer
| Autor: | Cigir Biray Avci, Cansu Caliskan Kurt, Nur Selvi Günel, Bakiye Goker Bagca, Neslihan Pinar Ozates, Fatma Sogutlu, Behrouz Shademan, Nihal Birden |
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| Rok vydání: | 2021 |
| Předmět: |
Squamous-Cell Carcinoma
Proliferation Thyroid Gland Gene Expression Down-Regulation Expression Apoptosis Thyroid Carcinoma Anaplastic Models Biological Histone Deacetylases Anaplastic thyroid cancers Microrna Papillary thyroid cancer Invasion Cell Line Tumor Biomarkers Tumor Valproic acid Genetics medicine Humans Endocrine system Histone deacetylase Anaplastic thyroid cancer Follicular thyroid cancer Molecular Biology Thyroid cancer Migration miRNA Valproic Acid Targets business.industry Gene Expression Profiling Thyroid General Medicine medicine.disease Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure Cancer research lipids (amino acids peptides and proteins) Transcriptome business medicine.drug |
| Zdroj: | Molecular Biology Reports. 48:6085-6091 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-021-06616-2 |
| Popis: | Background Thyroid cancer is the most common malignant tumor of the endocrine system seen in the thyroid gland. More than 90% of thyroid cancers comprise papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC). Although anaplastic thyroid carcinoma (ATC) accounts for less than 2% of thyroid cancer. But patients' lifespan after diagnosis is about 6 months. Surgical interventions, radioactive iodine use, and chemotherapy are not sufficient in the treatment of ATC, so alternative therapies are needed. Methods and results The WST-1 assay test was performed to evaluate the anti-proliferative effects of Valproic acid (VPA). Also, the effect of VPA on miRNAs affecting histone deacetylase was determined by Quantitative RT-PCR. In the SW1736 cell line, IC50 dose for VPA was found 1.6 mg/ml. In our study, the level of oncogenic genes expression in cells treated with VPA, including miR-184, miR-222-5p, miR-124-3p, and miR-328-3p, decreased. Also, the expression of tumor inhibitory genes including miR-323-5p, miR-182-5p, miR-138-5p, miR-217, miR-15a-5p, miR-29b-3p, miR-324-5p and miR-101-5p increased significantly. Conclusions VPA can ad-just countless gene expression patterns, including microRNAs (miRNAs), by targeting histone deacetylase (HDAC). However, further studies are required for more accurate results. |
| Databáze: | OpenAIRE |
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