DNA methylation in the IGF2 intragenic DMR is re-established in a sex-specific manner in bovine blastocysts after somatic cloning
| Autor: | Doris Herrmann, Richard Reinhardt, Claudia Gebert, Heiner Niemann, Daniela Gröger, Andrea Lucas-Hahn, Joseph Wallace Carnwath, Janina Thiel, Hans Lehrach, Christine Wrenzycki, Petra Hajkova |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
animal structures Genomic imprinting Bovine embryos Parthenogenesis Bisulfite sequencing Fertilization in Vitro Biology Embryo Culture Techniques Andrology 03 medical and health sciences Sex Factors 0302 clinical medicine Insulin-Like Growth Factor II Somatic cloning Genetics medicine Animals Epigenetics Blastocyst 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine Reproduction Sequence Analysis DNA Methylation DNA Methylation Molecular biology Clone Cells 3. Good health Epigenetic reprogramming medicine.anatomical_structure embryonic structures DNA methylation Sex-specific DNA methylation Somatic cell nuclear transfer Cattle Female Reprogramming |
| Zdroj: | Genomics. 94:63-69 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2009.03.004 |
| Popis: | The recent identification of an intragenic differentially methylated region (DMR) within the last exon of the bovine Insulin-like growth factor 2 (IGF2) gene provides a diagnostic tool for in-depth investigation of bovine imprinting and regulatory mechanisms which are active during embryo development. Here, we used bisulfite sequencing to compare sex-specific DNA methylation patterns within this DMR in bovine blastocysts produced in vivo, by in vitro fertilization and culture, SCNT, androgenesis or parthenogenesis. In in vivo derived embryos, DNA methylation was removed from this intragenic DMR after fertilization, but partially replaced by the time the embryo reached the blastocyst stage. Among embryos developing in vivo, the level of DNA methylation was significantly lower in female than in male blastocysts. This sexual dimorphism was also found between parthenogenetic and androgenetic embryos, and followed the donor cell sex in SCNT derived blastocysts and is evidence for correct methylation reprogramming in SCNT embryos. |
| Databáze: | OpenAIRE |
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