Building a better biofilm - formation of in vivo-like biofilm structures by Pseudomonas aeruginosa in a porcine model of cystic fibrosis lung infection
| Autor: | Freya Harrison, Esther Sweeney, Niamh E. Harrington |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
lcsh:Biotechnology
030303 biophysics lcsh:QR1-502 Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Cystic fibrosis lcsh:Microbiology Article 03 medical and health sciences In vivo lcsh:TP248.13-248.65 medicine Molecular Biology Pathogen 030304 developmental biology 0303 health sciences 030306 microbiology Pseudomonas aeruginosa Biofilm Cell Biology biochemical phenomena metabolism and nutrition Antimicrobial medicine.disease 3R’s In vitro 3. Good health QR Chronic infection ex vivo model Ex vivo RC |
| Zdroj: | Biofilm Biofilm, Vol 2, Iss, Pp 100024-(2020) |
| ISSN: | 2590-2075 |
| DOI: | 10.1101/858597 |
| Popis: | Pseudomonas aeruginosa biofilm infections in the cystic fibrosis (CF) lung are highly resistant to current antimicrobial treatments and are associated with increased mortality rates. The existing models for such infections are not able to reliably mimic the clinical biofilms observed. We aimed to further optimise an ex vivo pig lung (EVPL) model for P. aeruginosa CF lung infection that can be used to increase understanding of chronic CF biofilm infection. The EVPL model will facilitate discovery of novel infection prevention methods and treatments, and enhanced exploration of biofilm architecture. We investigated purine metabolism and biofilm formation in the model using transposon insertion mutants in P. aeruginosa PA14 for key genes: purD, gacA and pelA. Our results demonstrate that EVPL recapitulates a key aspect of in vivo P. aeruginosa infection metabolism, and that the pathogen forms a biofilm with a clinically realistic structure not seen in other in vitro studies. Two pathways known to be required for in vivo biofilm infection - the Gac regulatory pathway and production of the Pel exopolysaccharide - are essential to the formation of this mature, structured biofilm on EVPL tissue. We propose the high-throughput EVPL model as a validated biofilm platform to bridge the gap between in vitro and CF lung infection. |
| Databáze: | OpenAIRE |
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