Correction: In silico guided structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: A functional marker based approach
| Autor: | Geleta Dugassa Barka, Laércio Zambolim, Sávio Siqueira Ferreira, Eveline Teixeira Caixeta |
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| Přispěvatelé: | GELETA DUGASSA BARKA, UNIVERSIDADE FEDERAL DE VIÇOSA, EVELINE TEIXEIRA CAIXETA MOURA, CNPCa, SÁVIO SIQUEIRA FERREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA, LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA. |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Candidate gene Molecular biology Artificial Gene Amplification and Extension 01 natural sciences Genome Coffee Polymerase Chain Reaction Conserved sequence Solanum lycopersicum Vitis Phylogeny Disease Resistance Plant Proteins Data Management Genetics Molecular breeding Multidisciplinary biology food and beverages Phylogenetic Analysis Genomics Phylogenetics BAC cloning Medicine Cafezal Resistência Ferrugem Genome Plant Research Article Computer and Information Sciences Virulence Factors Hemileia Vastatrix Gene prediction Science Research and Analysis Methods 03 medical and health sciences Open Reading Frames Vector cloning Evolutionary Systematics Amino Acid Sequence Molecular Biology Techniques Gene Prediction Gene Gene Library Plant Diseases Taxonomy Hemileia vastatrix Evolutionary Biology Binding Sites Polymorphism Genetic Biology and life sciences Basidiomycota Correction Computational Biology Comparative Genomics biology.organism_classification Genome Analysis 030104 developmental biology Genetic Loci Sequence Alignment 010606 plant biology & botany Cloning |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 9, p e0238967 (2020) Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA PLoS ONE, Vol 15, Iss 7, p e0222747 (2020) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0238967 |
| Popis: | Physiology-based differentiation of SH genes and Hemileia vastatrix races is the principal method employed for the characterization of coffee leaf rust resistance. Based on the gene-for-gene theory, nine major rust resistance genes (SH1-9) have been proposed. However, these genes have not been characterized at the molecular level. Consequently, the lack of molecular data regarding rust resistance genes or candidates is a major bottleneck in coffee breeding. To address this issue, we screened a BAC library with resistance gene analogs (RGAs), identified RGAs, characterized and explored for any SH related candidate genes. Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. Furthermore, comparative analysis of the two RGAs belonging to CC-NBS-LRR revealed more intense diversifying selection in tomato and grape genomes than in coffee. For the first time, the present study has unveiled novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding. The characterized candidate RGA is of particular importance for further biological function analysis in coffee. Made available in DSpace on 2020-07-15T11:11:10Z (GMT). No. of bitstreams: 1 in-silico-guided-structural.pdf: 2828975 bytes, checksum: 122284f694e73c0616a4956d5037e108 (MD5) Previous issue date: 2020 |
| Databáze: | OpenAIRE |
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