Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas
| Autor: | Ines Drenjančević, Brigita Popović, Zdenko Lončarić, Krunoslav Karalić, Ana Stupin, Ivana Jukić, Monika Vesel, Sanja Novak, Anita Ćosić |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male GPX1 medicine.medical_specialty endothelium Thiobarbituric acid Health Toxicology and Mutagenesis 030204 cardiovascular system & hematology medicine.disease_cause Arginine Nitric Oxide Article Gene Expression Regulation Enzymologic Nitric oxide vascular function Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Selenium 0302 clinical medicine Internal medicine medicine TBARS Animals Aorta biology selenium oxidative stress rats Public Health Environmental and Occupational Health Ferric reducing ability of plasma Animal Feed Diet Rats Nitric oxide synthase Oxidative Stress 030104 developmental biology Endocrinology Biochemistry chemistry Catalase biology.protein Endothelium Vascular Oxidative stress |
| Zdroj: | International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health; Volume 14; Issue 6; Pages: 591 |
| ISSN: | 1660-4601 1661-7827 |
| Popis: | This study aimed to determine whether low dietary Se content affects the function and mechanisms mediating the vascular relaxation of rat aortas, and to test the role of oxidative stress in observed differences. Male Sprague Dawley (SD) rats were maintained for 10 weeks on low Se (low-Se group; N = 20) or normal Se content (norm-Se group; N = 20) rat chow. Dose responses to acetylcholine (ACh; 10−9–10−5M) and the response to reduced pO2 were tested in noradrenaline-precontracted aortic rings in the absence/presence of the nitric oxide synthase (NOS) inhibitor nitro-l-arginine methyl ester (l-NAME), the cyclooxygenase 1 and 2 (COX-1, 2) inhibitor Indomethacin, and the antioxidative agent Tempol in tissue bath. mRNA expression of glutathione peroxidase 1 (GPx1), catalase (CAT), and Cu/Zn superoxide dismutase (SOD) was measured in rat aortas. Oxidative stress (Thiobarbituric Acid Reactive Substances; TBARS), antioxidative plasma capacity (ferric reducing ability of plasma assay; FRAP), and protein levels of GPx1 were measured in plasma and serum samples, respectively. Reduced ACh-induced relaxation (AChIR) (dominantly mediated by NO) in the low-Se group compared to the norm-Se group was restored by Tempol administration. Hypoxia-induced relaxation (HIR) (dominantly mediated by COX-1, 2), TBARS, and FRAP as well as GPx1 serum concentrations were similar between the groups. mRNA GPx1 expression in rat aortas was significantly decreased in the low-Se compared to the norm-Se group. These data suggest that low dietary Se content increases the local oxidative stress level, which subsequently affects the NO-mediated vascular response. |
| Databáze: | OpenAIRE |
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