Loss of Protein Kinase Cδ from Human HaCaT Keratinocytes upon Ras Transfection Is Mediated by TGFα
| Autor: | Michael Gschwendt, Detlef Geiges, Friedrich Marks |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Keratinocytes
Human skin Transfection Isozyme Gene Expression Regulation Enzymologic Lactones chemistry.chemical_compound Cytosol Humans RNA Messenger Bryostatin Protein kinase A Protein Kinase C Protein kinase C Cell Line Transformed integumentary system biology Cell Biology Bryostatins Molecular biology Cell biology Isoenzymes HaCaT Genes ras chemistry Culture Media Conditioned biology.protein Tetradecanoylphorbol Acetate Electrophoresis Polyacrylamide Gel Macrolides Enzyme Repression Antibody |
| Zdroj: | Experimental Cell Research. 219:299-303 |
| ISSN: | 0014-4827 |
| Popis: | The spontaneously immortalized human skin keratinocytes HaCaT contain protein kinase C (PKC) alpha, -delta, -epsilon, and -zeta. All PKC isoenzymes except PKC zeta are down-regulated by TPA as well as by bryostatin. However, with PKC delta, bryostatin but not TPA was found to be much less effective at high concentrations than at low ones. PKC delta expression at the protein and mRNA level is significantly suppressed in HaCaT cells I-7 and II-4, which are transfected with mutated c-Ha-ras. The expression of the other isoenzymes remains essentially unchanged in the ras-transfected cells compared to normal ones. PKC delta is lost when growing HaCaT cells in a medium obtained from the cultivation of ras-transfected cells ("ras-conditioned" medium). The factor secreted into the medium by the ras-transfected cells that is responsible for this effect appears to be TGF alpha, since the action of ras-conditioned medium on PKC delta expression can be overcome by the addition of an anti-TGF alpha antibody. Moreover, treatment of HaCaT cells with TGF alpha suppresses selectively the expression of the PKC isoenzyme delta. |
| Databáze: | OpenAIRE |
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