Serum immunoreactive erythropoietin and red blood cell mass during pregnancy in conscious rats
| Autor: | Kirk P. Conrad, G. O. Del Valle, M. D. Mosher |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Time Factors Physiology Radioimmunoassay Blood volume Hematocrit Biology Pregnancy Physiology (medical) Internal medicine medicine Animals Erythropoietin Erythrocyte Volume Analysis of Variance medicine.diagnostic_test Rats Inbred Strains medicine.disease Rats Red blood cell Endocrinology medicine.anatomical_structure Gestation Pregnancy Animal Regression Analysis Female Analysis of variance medicine.drug |
| Zdroj: | The American journal of physiology. 265(2 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | Serum erythropoietin concentration increases during human pregnancy and presumably accounts for expansion of red blood cell mass. The mechanism(s) underlying gestational changes of serum erythropoietin are unknown. Moreover, if erythropoietin synthesis increases, then the organ(s) questions about erythropoietin in pregnancy, we first set out to establish an animal model. Chronically instrumented, conscious unrestrained rats were studied. 51Cr-labeled red blood cells and radioimmunoassay were used to assess red blood cell mass and serum erythropoietin, respectively. Except for a lower hematocrit (P < 0.05 vs. virgin rats) and a slightly higher plasma volume (P value not significant) for gravid rats on gestational day 6, all other variables measured in early pregnancy rats were comparable to those measured in virgin control animals. Significant increases in total blood volume, plasma volume, and red blood cell mass were observed by gestational day 13 (midpregnancy) when compared with virgin control rats. These changes were even more pronounced on gestational day 20. Serum immunoreactive erythropoietin was also significantly increased at both of these stages of pregnancy. We conclude that the gravid rat is a reliable animal model of human gestation in which to further investigate erythropoietin in pregnancy. |
| Databáze: | OpenAIRE |
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