Anisotropic poly(lactic-co-glycolic acid) microparticles enable sustained release of a peptide for long-term inhibition of ocular neovascularization
| Autor: | Raquel Lima e Silva, Adam C. Mirando, Elana Ben-Akiva, Ron B. Shmueli, Peter A. Campochiaro, Jordan J. Green, Niranjan B. Pandey, Jayoung Kim, Aleksander S. Popel, Stephany Y. Tzeng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
genetic structures
0206 medical engineering Biomedical Engineering Mice Transgenic 02 engineering and technology Pharmacology Biochemistry Article Biomaterials Neovascularization Mice chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Biomimetic Materials In vivo medicine Animals Microparticle Molecular Biology Retinal General Medicine Intravitreal administration Exudative retinal detachment 021001 nanoscience & nanotechnology 020601 biomedical engineering Choroidal Neovascularization eye diseases PLGA RAW 264.7 Cells Choroidal neovascularization chemistry Delayed-Action Preparations Intravitreal Injections Anisotropy sense organs medicine.symptom Peptides 0210 nano-technology Biotechnology |
| Zdroj: | Acta Biomater |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2019.07.054 |
| Popis: | Leading causes of vision loss include neovascular age-related macular degeneration (NVAMD) and macular edema (ME), which both require frequent intravitreal injections for treatment. A safe, poly(lactic-co-glycolic acid) (PLGA)-based biodegradable polymeric microparticle (MP) delivery system was developed that encapsulates and protects a biomimetic peptide from degradation, allows sustained intraocular release through polymer hydrolysis, and demonstrates a prolonged anti-angiogenic effect in vivo in three different NVAMD animal models (a laser-induced choroidal neovascularization mouse model, a rhoVEGF transgenic mouse model, and a Tet/opsin/VEGF transgenic mouse model) following intravitreal administration. The role of copolymer composition and microparticle shape was explored and 85:15 lactide-to-glycolide PLGA formed into ellipsoidal microparticles was found to be effective at inhibiting neovascularization for at least 16 weeks in vivo. Treatments were found to not only inhibit the growth of neovascularization, but also to cause regression of the neovasculature, reduce vascular leakage, and prevent exudative retinal detachment. These particulate devices are promising for the sustained release of biologics in the eye and may be useful for treating retinal diseases. Statement of Significance Devastating retinal diseases cause blindness in millions of people around the world. Current protein-based treatments have insufficient efficacy for many patients and also necessitate frequent intravitreal injections. Here, we demonstrate a new treatment consisting of a peptide encapsulated in biodegradable microparticles. We explore the effects of copolymer composition and physical shape of polymeric microparticles and find that both modulate peptide release. Efficacy of the treatment was validated in three different mouse models and the lead formulation was determined to be effective long-term, for at least 16 weeks in vivo, following a single injection. Treatments inhibited and regressed neovascularization as well as reduced vascular leakage. Anisotropic polymeric microparticles are promising for the sustained release of biologics in the eye. |
| Databáze: | OpenAIRE |
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