Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism
| Autor: | Sandrine Auger, Isabelle Guillouard, Antoine Danchin, Pierre Burguière, Isabelle Martin-Verstraete, Juliette Fert |
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| Přispěvatelé: | Génétique des Génomes Bactériens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This research was supported by grants from the 'Ministère de l'Education Nationale de la Recherche et de la Technologie,' the 'Centre National de la Recherche Scientifique' (URA 2171), the 'Institut Pasteur,' the 'Université Paris 7,' the 'Fondation pour la recherche médicale,' and the European Biotech Program (contract QLG2 CT9901455)., Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] |
| Rok vydání: | 2005 |
| Předmět: |
Genotype
Transcription Genetic Operon Molecular Sequence Data Restriction Mapping Repressor Genetics and Molecular Biology Bacillus subtilis Biology Microbiology trp operon 03 medical and health sciences Bacterial Proteins gal operon Molecular Biology Gene 030304 developmental biology Regulator gene 0303 health sciences Base Sequence 030306 microbiology GENE EXPRESSION REGULATION ABC TRANSPORTER Promoter biology.organism_classification SEQUENCE ANALYSIS Phosphotransferases (Alcohol Group Acceptor) SULFUR METABOLISM [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biochemistry Mutagenesis Site-Directed Cystine ATP-Binding Cassette Transporters Sulfur |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, 2005, 87 (17), pp.6019-6030. ⟨10.1128/JB.187.17.6019-6030.2005⟩ Journal of Bacteriology, American Society for Microbiology, 2005, 87 (17), pp.6019-6030. ⟨10.1128/JB.187.17.6019-6030.2005⟩ |
| ISSN: | 0021-9193 1098-5530 |
| Popis: | The YtlI regulator of Bacillus subtilis activates the transcription of the ytmI operon encoding an l -cystine ABC transporter, a riboflavin kinase, and proteins of unknown function. The expression of the ytlI gene and the ytmI operon was high with methionine and reduced with sulfate. Using deletions and site-directed mutagenesis, a cis -acting DNA sequence important for YtlI-dependent regulation was identified upstream from the −35 box of ytmI . Gel mobility shift assays confirmed that YtlI specifically interacted with this sequence. The replacement of the sulfur-regulated ytlI promoter by the xylA promoter led to constitutive expression of a ytmI ′ -lacZ fusion in a ytlI mutant, suggesting that the repression of ytmI expression by sulfate was mainly at the level of YtlI synthesis. We further showed that the YrzC regulator negatively controlled ytlI expression while this repressor also acted on ytmI expression via YtlI. The cascade of regulation observed in B. subtilis is conserved in Listeria spp. Both a YtlI-like regulator and a ytmI -type operon are present in Listeria spp. Indeed, the Lmo2352 protein from Listeria monocytogenes was able to replace YtlI for the activation of ytmI expression and a lmo2352′ -lacZ fusion was repressed in the presence of sulfate via YrzC in B. subtilis . A common motif, AT(A/T)ATTCCTAT, was found in the promoter region of the ytlI and lmo2352 genes. Deletion of part of this motif or the introduction of point mutations in this sequence confirmed its involvement in ytlI regulation. |
| Databáze: | OpenAIRE |
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