Hydrogen sulfide biosynthesis is impaired in the osteoarthritic joint
| Autor: | Francisco J. Blanco, Rosa Meijide-Faílde, Elena F. Burguera, L. Gato-Calvo, Carlos Vaamonde-García, Á. Vela-Anero |
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| Rok vydání: | 2019 |
| Předmět: |
Serum
Sulfurous thermal waters Atmospheric Science medicine.medical_specialty 010504 meteorology & atmospheric sciences Health Toxicology and Mutagenesis Arthritis Cystathionine beta-Synthase Endogeny Osteoarthritis Mitochondrion 01 natural sciences 03 medical and health sciences 0302 clinical medicine Western blot Internal medicine medicine Humans Hydrogen Sulfide 0105 earth and related environmental sciences Aged 030203 arthritis & rheumatology chemistry.chemical_classification Hydrogen sulfide Ecology biology medicine.diagnostic_test Chemistry Cartilage Cystathionine gamma-Lyase Human articular cartilage medicine.disease Cystathionine beta synthase Mitochondria Endocrinology medicine.anatomical_structure Enzyme biology.protein |
| Zdroj: | RUC: Repositorio da Universidade da Coruña Universidade da Coruña (UDC) RUC. Repositorio da Universidade da Coruña Universitat Oberta de Catalunya (UOC) |
| ISSN: | 1432-1254 |
| Popis: | [Abstract] Osteoarthritis (OA) is the most common form of arthritis and it is a leading cause of disability in the elderly. Its complete etiology is not known although there are several metabolic, genetic, epigenetic, and local contributing factors involved. At the moment, there is no cure for this pathology and treatment alternatives to retard or stop its progression are intensively being sought. Hydrogen sulfide (H2S) is a small gaseous molecule and is present in sulfurous mineral waters as its active component. Data from recent clinical trials shows that balneotherapy (immersion in mineral and/or thermal waters from natural springs) in sulfurous waters can improve OA symptoms, in particular, pain and function. Yet, the underlying mechanisms are poorly known. Hydrogen sulfide is also considered, with NO and CO, an endogenous signaling gasotransmitter. It is synthesized endogenously with the help of three enzymes, cystathionine gamma-lyase (CTH), cystathionine beta-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (3-MPST). Here, the expression of these three enzymes was demonstrated by quantitative real-time polymerase chain reaction (qRT-PCR) and their protein abundance [by immunohistochemistry and Western blot (WB)] in human articular cartilage. No significant differences were found in CBS or CTH expression or abundance, but mRNA and protein levels of 3-MPST were significantly reduced in cartilage form OA donors. Also, the biosynthesis of H2S from OA cartilage, measured with a specific microelectrode, was significantly lower than in OA-free tissue. Yet, no differences were found in H2S concentration in serum from OA patients and OA-free donors. The current results suggest that reduced levels of the mitochondrial enzyme 3-MPST in OA cartilage might be, at least in part, responsible for a reduction in H2S biosynthesis in this tissue and that impaired H2S biosynthesis in the joint might be a contributing factor to OA. This could contribute to explain why exogenous supplementation of H2S, for instance with sulfurous thermal water, has positive effects in OA patients. Instituto de Salud carlos III; PI12/00329 Instituto de Salud Carlos III; PI16/02124 Instituto de Salud Carlos III; RETIC-RIER-RD12/0009/0018 Xunta de Galicia; IN607A 2017/11 |
| Databáze: | OpenAIRE |
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