The dose-dependent effects of endotoxin on protein metabolism in two types of rat skeletal muscle
| Autor: | Miroslav Kovarik, Tomas Muthny, Milan Holecek, Ludek Sispera |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Proteasome Endopeptidase Complex Physiology Proteolysis Protein metabolism Muscle Proteins Biology Biochemistry Cathepsin B Sepsis chemistry.chemical_compound Myofibrils Internal medicine medicine Animals Rats Wistar Muscle Skeletal chemistry.chemical_classification medicine.diagnostic_test Triglyceride Dose-Response Relationship Drug Skeletal muscle General Medicine medicine.disease Amino acid Rats Endotoxins Protein catabolism Endocrinology medicine.anatomical_structure chemistry Myofibril |
| Zdroj: | Journal of physiology and biochemistry. 68(3) |
| ISSN: | 1877-8755 |
| Popis: | Endotoxin administration is frequently used as a model of systemic inflammatory response which is considered the important pathogenetic factor in muscle wasting development in severe illness, such as sepsis, cancer, injury, AIDS and others. The main purpose of this study was determining the effect of various doses of endotoxin on protein and amino acid metabolism in two types of rat skeletal muscle. Sepsis was induced by intraperitoneal administration of endotoxin in a dose of 1, 3 and 5 mg/kg body weight (bw); control animals received a corresponding volume of the saline solution. After 24 h, extensor digitorum longus (EDL) and soleus (SOL) muscles were isolated and used for determination of total and myofibrillar proteolysis, protein synthesis, activity of cathepsins B and L, chymotrypsin-like activity of proteasome and amino acid release. The endotoxemia induced the body weight loss, the rise of total cholesterol and triglyceride plasma concentration and the protein catabolic state in skeletal muscle, which was caused by a higher increase in protein breakdown (due to activation of the proteasome system) than protein synthesis. The more significant effect of endotoxin was seen in EDL than SOL. The dose of 5 mg of endotoxin/kg bw induced the most significant changes in parameters of the protein and amino acid metabolism measured and could be therefore considered appropriate for studies of protein catabolism in young rat skeletal muscle at 24 h after endotoxin treatment. |
| Databáze: | OpenAIRE |
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