Extracts of Chilean native fruits inhibit oxidative stress, inflammation and insulin-resistance linked to the pathogenic interaction between adipocytes and macrophages
| Autor: | L. Garcia, Claudia Parra-Ruiz, M.T. Pino, Karla Vasquez, Angelica Ovalle-Marin, O. Zamora, Diego F. Garcia-Diaz, V. Quitral, Marjorie Reyes-Farias, F. Fuentes, Paula Jimenez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Antioxidant medicine.medical_treatment Glucose uptake Medicine (miscellaneous) Adipose tissue Inflammation Biology medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound Insulin resistance Internal medicine medicine Adipocytes TX341-641 Obesity Nutrition and Dietetics Nutrition. Foods and food supply Macrophages Glutathione Insulin-resistance medicine.disease In vitro 030104 developmental biology Endocrinology chemistry Biochemistry Oxidative stress medicine.symptom Food Science |
| Zdroj: | Journal of Functional Foods, Vol 27, Iss, Pp 69-83 (2016) |
| ISSN: | 1756-4646 |
| Popis: | Obesity-associated insulin-resistance is set by a chronic inflammatory state established in the adipose tissue. Chilean native fruits calafate (CA) and maqui (MA) berries present remarkable anti-inflammatory features. Here, we evaluated antioxidant, anti-inflammatory and insulin-sensitizer effects of these fruits in an in vitro inflammatory setting. Differentiated 3T3-L1 cells exposed to conditioned media (CM) from activated macrophages were treated with CA and MA extracts. MA increased metalloproteinase (MMP)-2 activity on day 3, and both CA and MA modulated MMP-9 activity on day 10 of differentiation. In differentiated CM-treated 3T3-L1, extracts increased GSH levels and GSH/GSSG ratio, CA and MA prevented caspase-3 induction, and MA decreased MCP-1, while CA increased IL-6 gene expressions. Finally, MA reverted CM specific IRS-1 phosphorylation, and CA improved insulin-stimulated glucose uptake. Thus, treatments with extracts of Chilean native fruits were able to block the development of oxidative stress, inflammation and insulin-resistance in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect