Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1
| Autor: | Lei Jiang, Weijian Zhu, Xuecheng Sun, Shuhan Li, Daoquan Fang, Wenqi Zhang, Feiyan Lin, Lifang Luo, Wenjing Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Colorectal cancer colorectal cancer exosomes miR-335-5p RM1-950 Biology Exosome Metastasis 03 medical and health sciences 0302 clinical medicine Drug Discovery microRNA medicine metastasis neoplasms Cell migration medicine.disease Microvesicles digestive system diseases 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Cancer cell Cancer research Molecular Medicine Original Article Therapeutics. Pharmacology RASA1 |
| Zdroj: | Molecular Therapy: Nucleic Acids, Vol 24, Iss, Pp 164-174 (2021) Molecular Therapy. Nucleic Acids |
| ISSN: | 2162-2531 |
| Popis: | Exosomal microRNA (miRNA) secretion has been characterized as a vital factor in intercellular communication among cancer cells. However, little is known about cancer-secreted miRNAs specifically involved in metastasis of colorectal cancer (CRC). Here, we found that exosomes derived from metastatic CRC cell line SW620 promoted migration, invasion, and epithelial-mesenchymal transition (EMT) of CRC cells. The profiling of exosome miRNAs revealed that microRNA (miR)-335-5p was highly expressed in exosomes from metastatic SW620 cells compared to those derived from primary SW480 cells. miR-335-5p was transmitted from metastatic SW620 cells to CRC cells via exosomes and promoted migration, invasion, and EMT of CRC cells. Moreover, exosome-transmitted miRNA-335-5p promotes CRC cell invasion and metastasis by facilitating EMT via targeting RAS p21 protein activator 1 (RASA1). Overexpression of RASA1 abolished the promotive effects of exosomal miR-335-5p on CRC cell migration, invasion, and EMT. Collectively, our data revealed that exosomal miR-335-5p derived from metastatic CRC cells promotes CRC cell invasion and metastasis by facilitating EMT via targeting RASA1, which may serve as a potential therapeutic target for CRC metastasis. Graphical Abstract In this study, these data revealed that exosomal miR-335-5p derived from metastatic CRC cells promoted CRC cell invasion and metastasis by facilitating EMT via targeting RASA1, which may serve as a potential therapeutic target for CRC metastasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|