Crocin induces anti-ischemia in middle cerebral artery occlusion rats and inhibits autophagy by regulating the mammalian target of rapamycin
| Autor: | Haoxin Wu, Haoran Wu, Ruizhi Jiang, Qinan Wu, Ke Bao, Xin Shan, Guangda Sun, Jie Xu, Zhiheng Huang, Xiaoyan Huang, Weiwei Tao |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cell Survival Ischemia Down-Regulation Pharmacology Crocin Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot In vivo medicine Autophagy Animals Protein kinase A PI3K/AKT/mTOR pathway medicine.diagnostic_test business.industry TOR Serine-Threonine Kinases Infarction Middle Cerebral Artery medicine.disease Carotenoids Rats 030104 developmental biology Mechanism of action chemistry medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | European journal of pharmacology. 857 |
| ISSN: | 1879-0712 |
| Popis: | Crocin, an active compound found in Gardenia jasminoides Ellis, has been shown to possess neuron-protective properties, but its potential mechanisms of action still remain poorly understood. In this study, the anti-ischemic effect and underlying mechanism of action of crocin were investigated in male rats with right middle cerebral artery occlusion/reperfusion. Computed tomography and magnetic resonance imaging were used to evaluate the area of infarction 24 h after reperfusion. Neurological scores were employed to evaluate nerve injury. Direct 2,3,5-triphenyltetrazolium chloride staining was used to calculate the infarct ratio 120 h after reperfusion. Finally, HT22 cells and Western blot were used to study the underlying mechanisms. Crocin showed a decreased infarct volume and neurological score in vivo, while the expression of LC3-II/I and AMP-activated protein kinase was remarkably down-regulated with increased levels of p62 and mammalian target of rapamycin (mTOR) expression. However, rapamycin significantly inhibited mTOR, which can impact the anti-ischemic effect of crocin in vitro. These results suggest that crocin may elicit an anti-ischemic effect probably through the mTOR pathway. |
| Databáze: | OpenAIRE |
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